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AB251471

Anti-BMP9 antibody [EPR20009] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal BMP9 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BMP9, GDF2, Growth/differentiation factor 2, GDF-2, Bone morphogenetic protein 9, BMP-9

2 Images
Western blot - Anti-BMP9 antibody [EPR20009] - BSA and Azide free (AB251471)
  • WB

Supplier Data

Western blot - Anti-BMP9 antibody [EPR20009] - BSA and Azide free (AB251471)

This data was developed using ab207318, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

BMP9 is mostly expressed in liver and has very low expression levels in brain and other tissues (PMID 21710321; 23812757).

All lanes:

Western blot - Anti-BMP9 antibody [EPR20009] (<a href='/en-us/products/primary-antibodies/bmp9-antibody-epr20009-ab207318'>ab207318</a>) at 1/1000 dilution

Lane 1:

Human liver lysate at 20 µg

Lane 2:

Human brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Exposure time: 3min

Western blot - Anti-BMP9 antibody [EPR20009] - BSA and Azide free (AB251471)
  • WB

Supplier Data

Western blot - Anti-BMP9 antibody [EPR20009] - BSA and Azide free (AB251471)

This data was developed using ab207318, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-BMP9 antibody [EPR20009] (<a href='/en-us/products/primary-antibodies/bmp9-antibody-epr20009-ab207318'>ab207318</a>) at 1/10000 dilution

Lane 1:

GST-tagged Human BMP9 recombinant protein (aa338-429) at 0.02 µg

Lane 2:

His-tagged Human BMP10 recombinant protein (aa334-424) at 0.02 µg

Lane 3:

His-tagged Human BMP13 recombinant protein (aa365-455) at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 35 kDa

false

Exposure time: 4s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20009

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251471 is the carrier-free version of ab207318.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.
See full target information GDF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1180 PubMed40775066

2025

Regulation of cortical neurogenesis by MED13L via transcriptional priming and its implications for MED13L syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Li,Yu-Zhu Hao,Jia Cui,Xiao-Xiao Cao,Nan Wu,Ji Ma,Shuang-Yi Xie,Yi-Hsuan Pan,Xue-Lian He,Yu-Lan Zhao,Xiao-Bing Yuan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com