AB78417

Anti-BMPR1B antibody [MM0055-3E12]

Name: Anti-BMPR1B antibody [MM0055-3E12] (ab78417) | Abcam
Brand: Abcam Limited
SKU: AB78417
Price: 520 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal BMPR1B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Bone morphogenetic protein receptor type-1B.

View Alternative Names

CDw293, Bone morphogenetic protein receptor type-1B, BMP type-1B receptor, BMPR-1B, BMPR1B

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR1B antibody [MM0055-3E12] (AB78417)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR1B antibody [MM0055-3E12] (AB78417)

ab78417 at 1/200 dilution staining BMPR1B in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Unknown

Western blot - Anti-BMPR1B antibody [MM0055-3E12] (AB78417)

The band observed at 53 kDa could potentially be a cleaved form of BMPR1B due to presence of a 13 amino acid signal peptide.

All lanes:

Western blot - Anti-BMPR1B antibody [MM0055-3E12] (ab78417) at 1 µg/mL

Lane 1:

Human bone tumor tissue lysate - total protein (<a href='/en-us/products/unavailable/human-bone-tumor-tissue-lysate-total-protein-ab29359'>ab29359</a>) at 10 µg

Lane 2:

WI38 (Human lung fibroblast cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 57 kDa

Observed band size: 30 kDa,37 kDa,50 kDa,57 kDa

true

Exposure time: 30s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0055-3E12

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Bone morphogenetic protein receptor type-1B.

O00238

Specificity

ab78417 detects BMPR1B. No cross reactivity was found to BMPR1A (ALK3).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution inphosphate buffered saline (PBS).

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. Positively regulates chondrocyte differentiation through GDF5 interaction.

See full target information Bone morphogenetic protein receptor type-1B

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 23:599-614.e4 PubMed30174296

2018

Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Wang,Xixi Liu,Gang Chang,Yidong Chen,Geng An,Liying Yan,Shuai Gao,Yanwen Xu,Yueli Cui,Ji Dong,Yuhan Chen,Xiaoying Fan,Yuqiong Hu,Ke Song,Xiaohui Zhu,Yun Gao,Zhaokai Yao,Shuhui Bian,Yu Hou,Jiahao Lu,Rui Wang,Yong Fan,Ying Lian,Wenhao Tang,Yapeng Wang,Jianqiao Liu,Lianming Zhao,Luyu Wang,Zhaoting Liu,Renpei Yuan,Yujia Shi,Boqiang Hu,Xiulian Ren,Fuchou Tang,Xiao-Yang Zhao,Jie Qiao

Scientific reports 8:1561 PubMed29367682

2018

Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Lotta Tengroth,Julia Arebro,Olivia Larsson,Claus Bachert,Susanna Kumlien Georén,Lars-Olaf Cardell

Stem cells translational medicine 6:306-315 PubMed28170187

2017

A Selective Cell Population from Dermis Strengthens Bone Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Tingliang Wang,Jinguang He,Yang Zhang,Wenjun Shi,Jiasheng Dong,Ming Pei,Lian Zhu

Stem cell reports 4:239-54 PubMed25601208

2015

Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response.

Applications

IHC-P

Species

Human

Marion Chapellier,Elodie Bachelard-Cascales,Xenia Schmidt,Flora Clément,Isabelle Treilleux,Emmanuel Delay,Alexandre Jammot,Christine Ménétrier-Caux,Gaëtan Pochon,Roger Besançon,Thibault Voeltzel,Claude Caron de Fromentel,Christophe Caux,Jean-Yves Blay,Richard Iggo,Véronique Maguer-Satta

Development (Cambridge, England) 140:4751-62 PubMed24173804

2013

Growth differentiation factor 5 is a key physiological regulator of dendrite growth during development.

Applications

WB, ICC/IF

Species

Mouse, Mouse

Catarina Osório,Pedro J Chacón,Lilian Kisiswa,Matthew White,Sean Wyatt,Alfredo Rodríguez-Tébar,Alun M Davies

TheScientificWorldJournal 2012:467892 PubMed22272175

2012

Immunohistological localization of BMP-2, BMP-7, and their receptors in knee joints with focal cartilage lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Hagen Schmal,Alexander T Mehlhorn,Ingo H Pilz,David Dovi-Akue,Christina Kirchhoff,Norbert P Südkamp,Ulrike Gerlach,Christian Lohrmann,Philipp Niemeyer

The Journal of clinical endocrinology and metabolism 96:412-21 PubMed21084396

2010

Leiomyoma simultaneously impair endometrial BMP-2-mediated decidualization and anticoagulant expression through secretion of TGF-β3.

Applications

IHC-P

Species

Human

Donna C Sinclair,Alex Mastroyannis,Hugh S Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BMPR1B antibody [MM0055-3E12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR1B antibody [MM0055-3E12] (AB78417)

Western blot - Anti-BMPR1B antibody [MM0055-3E12] (AB78417)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise