Anti-BMPR2 antibody

• WB

Supplier Data

Western blot - Anti-BMPR2 antibody (AB96826)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-BMPR2 antibody (ab96826) at 1/5000 dilution

Lane 1:

A431 whole cell lysates at 30 µg

Lane 2:

HeLa whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 115 kDa

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• WB

CiteAb

Western blot - Anti-BMPR2 antibody (AB96826)

BMPR2 western blot using anti-BMPR2 antibody ab96826. Publication image and figure legend from Xu, Y., Luo, X., et al., 2018, Cell Death Dis, PubMed 30206204.

ab96826 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab96826 please see the product overview.

Cited1 interacts with Bmpr2 to activate BMP signaling and induce trophoblast differentiation.a Protein levels of pSmad1/5 and pSmad2 upon Cited1 overexpression in E14T ESCs at the times as indicated. Smad5, Smad2/3, and α-Tubulin were used as loading controls. b Protein levels of pSmad1/5 and pSmad2 upon Cited1 overexpression and inhibitor treatment for 3 days in E14T ESCs. Smad5, Smad2/3, and α-Tubulin were used as loading controls. c Bright field images of Cited1-overexpressing or control ESCs after treatment with DMSO or different inhibitors for 3 days. LDN193189 (0.1 μM), Noggin (400 ng/mL) and K02288 (10 μM) are all BMP signaling inhibitors. SB431542 (10 μM) is a TGF-β signaling inhibitor. d qRT-PCR analysis of transcript levels of trophoblast markers after transfection and treatment with inhibitors for 3 days. The average mRNA level in cells transfected with control vector pPy and treated with DMSO was set at 1.0. Data are shown as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. e qRT-PCR analysis of mRNA levels for ligands of the BMP pathway induced by Cited1 overexpression in E14T ESCs for 3 days. The average mRNA level in cells transfected with control vector pPy was set at 1.0. Data are shown as mean ± SD (n = 3). *p < 0.05. f The representative western blot result from Co-IP (immunoprecipitation) assays showing that ectopically expressed Flag-Cited1 binds to endogenous Bmpr2 specifically in E14T ESCs. Whole-cell lysates (WCL) were immunoprecipitated with anti-Flag M2 beads and analyzed by western blot with antibodies as indicated. Five percent of WCL was loaded as the input. g qRT-PCR analysis of mRNA levels of trophoblast specific markers in parental WT ESCs or Bmpr2 knockout (KO) cells upon Cited1 overexpression for 3 days. The average mRNA level in WT cells transfected with empty vector pPy was set at 1.0. Data are shown as mean ± SD (n = 3). ***p < 0.001

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