AB78422

Anti-BMPR2 antibody [MM0060-9A10]

Name: Anti-BMPR2 antibody [MM0060-9A10] (ab78422) | Abcam
Brand: Abcam Limited
SKU: AB78422
Price: 510 USD
Availability: InStock

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(13 Publications)

Mouse Monoclonal BMPR2 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BMPR2.

View Alternative Names

PPH1, BMPR2, Bone morphogenetic protein receptor type-2, BMP type-2 receptor, BMPR-2, Bone morphogenetic protein receptor type II, BMP type II receptor, BMPR-II

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

ab78422 at 1/200 dilution, staining BMPR2 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Flow Cytometry - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

Flow Cyt

Unknown

Flow Cytometry - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

Overlay histogram showing HepG2 cells stained with ab78422 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78422, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0060-9A10

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BMPR2.

Q13873

Specificity

ab78422 detects BMPR2. No cross reactivity was found to BMPR1A and BMPR1B.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^5 Cells", "FlowCyt-species-notes": "(methanol or paraformaldehyde fixed cells) <a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "We recommend to use Proteinase K for the enzymatic antigen retrieval." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline (PBS).

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Can also mediate signaling through the activation of the p38MAPK cascade (PubMed : 12045205). Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6. Promotes signaling also by binding to activin A/INHBA (PubMed : 24018044).

See full target information BMPR2

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37686014

2023

CRISPR-Cas9 Library Screening Identifies Novel Molecular Vulnerabilities in -Rearranged Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline Schneider,Priscilla Wander,Susan T C J M Arentsen-Peters,Kirsten S Vrenken,Dedeke Rockx-Brouwer,Fabienne R S Adriaanse,Veerle Hoeve,Irene Paassen,Jarno Drost,Rob Pieters,Ronald W Stam

Scientific reports 12:16310 PubMed36175474

2022

BMP2 promotes lung adenocarcinoma metastasis through BMP receptor 2-mediated SMAD1/5 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Kuei Wu,Man-Ting Wei,Hung-Chang Wu,Cheng-Lin Wu,Cheng-Ju Wu,Hungjiun Liaw,Wen-Pin Su

Bioengineered 13:8538-8547 PubMed35331081

2022

Lysine (K)-specific demethylase 5C regulates the incidence of severe preeclampsia by adjusting the expression of bone morphogenetic protein-7.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Feng Shi,Zhan Zhang,Hai-Ying Wu,Yu Wang,Ai-Min Chang,Jun-Jun Gao,Kan Liu,Wan-Yu Song,Li Wang,Huan-Ping Wang

Cell death & disease 10:261 PubMed30886151

2019

Osteosarcoma cell intrinsic PD-L2 signals promote invasion and metastasis via the RhoA-ROCK-LIMK2 and autophagy pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Ren,Bingxin Zheng,Yi Huang,Shidong Wang,Xing Bao,Kuisheng Liu,Wei Guo

Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 29:447-454 PubMed29142464

2017

BMPR2 and HIF1- overexpression in resected osteosarcoma correlates with distant metastasis and patient survival.

Applications

Unspecified application

Species

Unspecified reactive species

Shidong Wang,Tingting Ren,Yi Huang,Xing Bao,Kunkun Sun,Danhua Shen,Wei Guo

Oncotarget 8:58625-58641 PubMed28938584

2017

BMPR2 promotes invasion and metastasis via the RhoA-ROCK-LIMK2 pathway in human osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shidong Wang,Tingting Ren,Guangjun Jiao,Yi Huang,Xing Bao,Fan Zhang,Kuisheng Liu,Bingxin Zheng,Kunkun Sun,Wei Guo

Science signaling 9:ra58 PubMed27273096

2016

Augmented noncanonical BMP type II receptor signaling mediates the synaptic abnormality of fragile X syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Risa Kashima,Sougata Roy,Manuel Ascano,Veronica Martinez-Cerdeno,Jeanelle Ariza-Torres,Sunghwan Kim,Justin Louie,Yao Lu,Patricio Leyton,Kenneth D Bloch,Thomas B Kornberg,Paul J Hagerman,Randi Hagerman,Giorgio Lagna,Akiko Hata

Proceedings of the National Academy of Sciences of 112:E6882-8 PubMed26621735

2015

Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Jung Choi,Patrick N Ingram,Kun Yang,Lan Coffman,Mangala Iyengar,Shoumei Bai,Dafydd G Thomas,Euisik Yoon,Ronald J Buckanovich

Experimental biology and medicine (Maywood, N.J.) 240:1580-9 PubMed25854878

2015

Featured Article: microRNA-125a in pulmonary hypertension: Regulator of a proliferative phenotype of endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lars C Huber,Silvia Ulrich,Caroline Leuenberger,Max Gassmann,Johannes Vogel,Leonardo Glutz von Blotzheim,Rudolf Speich,Malcolm Kohler,Matthias Brock

Cell death & disease 5:e1571 PubMed25501832

2014

BMPR2 inhibition induced apoptosis and autophagy via destabilization of XIAP in human chondrosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

G Jiao,W Guo,T Ren,Q Lu,Y Sun,W Liang,C Ren,K Yang,K Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BMPR2 antibody [MM0060-9A10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

Flow Cytometry - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (13)

Product promise