JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151551

Anti-BNIP1 antibody [EPR10024]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal BNIP1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

NIP1, SEC20L, TRG8, BNIP1, Vesicle transport protein SEC20, BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, Transformation-related gene 8 protein, TRG-8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP1 antibody [EPR10024] (AB151551)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP1 antibody [EPR10024] (AB151551)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling BNIP1 with ab151551 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-BNIP1 antibody [EPR10024] (AB151551)
  • WB

Unknown

Western blot - Anti-BNIP1 antibody [EPR10024] (AB151551)

All lanes:

Western blot - Anti-BNIP1 antibody [EPR10024] (ab151551) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

L6 cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Lane 5:

Ramos cell lysate at 10 µg

Lane 6:

HepG2 cell lysate at 10 µg

Lane 7:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

  • Carrier free

    Anti-BNIP1 antibody [EPR10024] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10024

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB139486

Aggresome Detection Kit

Fluorescence Microscopy - Aggresome Detection Kit (AB139486)

  • 5
  • 1
Proteins & Peptides

AB202216

Recombinant Human BNIP1 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human BNIP1 protein (denatured) (His tag N-Terminus) (AB202216)

  • 0
  • 0
Sample Prep & Detection Kits

AB193970

Extraction Buffer 5X PTR

SimpleStep

  • 0
  • 0
Primary Antibodies

AB219465

Anti-6X His tag® antibody [RMH01]

Recombinant|Lab Essentials

Sandwich ELISA - Anti-6X His tag® antibody [RMH01] (AB219465)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization (PubMed : 15272311). Also plays a role in apoptosis (PubMed : 15272311, PubMed : 23896122, PubMed : 7954800). It is for instance required for endoplasmic reticulum stress-induced apoptosis (PubMed : 23896122). As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).
See full target information BNIP1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 51:e12402 PubMed29094413

2017

Fluoxetine induces autophagic cell death via eEF2K-AMPK-mTOR-ULK complex axis in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dejuan Sun,Lingjuan Zhu,Yuqian Zhao,Yingnan Jiang,Lixia Chen,Yang Yu,Liang Ouyang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com