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Rabbit Recombinant Monoclonal BNIP3 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

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Images

Western blot - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (AB219609), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (AB219609), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (AB219609), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (AB219609), expandable thumbnail
  • Western blot - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (AB219609), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

(Heat to 98°C, allow to cool for 10-20 minutes).

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

(Heat to 98°C, allow to cool for 10-20 minutes).

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

2 products for Alternative Version

Target data

Function

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.

Alternative names

NIP3, BNIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Recommended products

Rabbit Recombinant Monoclonal BNIP3 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4034
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab219609 is the carrier-free version of Anti-BNIP3 antibody [EPR4034] ab109362.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609), expandable thumbnail

    Western blot - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BNIP3 antibody [EPR4034] ab109362).

    Lane 1: Wild-type HAP1 whole cell lysate (40 μg)
    Lane 2: BNIP3 knockout HAP1 whole cell lysate (40 μg)
    Lane 3: SHSY5Y whole cell lysate (40 μg)
    Lane 4: A431 whole cell lysate (40 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-BNIP3 antibody [EPR4034] ab109362 observed at 25 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    Anti-BNIP3 antibody [EPR4034] ab109362 was shown to recognize BNIP3 in wild-type HAP1 cells as signal was lost at the expected MW in BNIP3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BNIP3 knockout samples were subjected to SDS-PAGE. Anti-BNIP3 antibody [EPR4034] ab109362 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-BNIP3 antibody [EPR4034] (Anti-BNIP3 antibody [EPR4034] ab109362)

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human renal adenocarcinoma tissue labelling BNIP3 with purified Anti-BNIP3 antibody [EPR4034] ab109362 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BNIP3 antibody [EPR4034] ab109362).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BNIP3 with unpurified Anti-BNIP3 antibody [EPR4034] ab109362 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BNIP3 antibody [EPR4034] ab109362).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609)

    Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling BNIP3 with unpurified Anti-BNIP3 antibody [EPR4034] ab109362 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BNIP3 antibody [EPR4034] ab109362).

  • Western blot - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609), expandable thumbnail

    Western blot - Anti-BNIP3 antibody [EPR4034] - BSA and Azide free (ab219609)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BNIP3 antibody [EPR4034] ab109362).

    The observed band sizes are consistent with publications. Bands around 30 and 60kDa should be monomer and dimer, respectively. Other bands should be different migrating forms (PMID: 15375294, PMID: 36396625, PMID: 9396766, PMID: 36396625, PMID: 33459136 etc.).
    1% SDS hot lysis method could help to get stronger monomer forms.

    The protocol used for this test is available under the Support & downloads section

    All lanes: Western blot - Anti-BNIP3 antibody [EPR4034] (Anti-BNIP3 antibody [EPR4034] ab109362) at 1/1000 dilution

    Lane 1: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20ug, prepared by RIPA method at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20ug, prepared by 1% SDS hot method at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 156s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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