AB155010

Anti-BNIP3L/NIX antibody

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(4 Publications)

Rabbit Polyclonal BNIP3L/NIX antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human BNIP3L aa 50-200.

View Alternative Names

BNIP3A, BNIP3H, NIX, BNIP3L, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody (AB155010)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody (AB155010)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling BNIP3L/NIX with ab155010 antibody at 1/200 dilution. Bottom figure : staining merged with DNA probe (Hoechst 33342).

Western blot - Anti-BNIP3L/NIX antibody (AB155010)

WB

Supplier Data

Western blot - Anti-BNIP3L/NIX antibody (AB155010)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-BNIP3L/NIX antibody (ab155010) at 1/1000 dilution

Lane 1:

Untreated HeLa whole cell extracts at 30 µg

Lane 2:

Treated HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

Immunogen

Synthetic Peptide within Human BNIP3L aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP : in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.

See full target information BNIP3L

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:3384-3410 PubMed40437287

2025

KEAP1 retention in phase-separated p62 bodies drives liver damage under autophagy-deficient conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Takada,Nozomi Shinomiya,Gaoxin Mao,Hikaru Tsuchiya,Tomoaki Koga,Satoko Komatsu-Hirota,Yu-Shin Sou,Manabu Abe,Elena Ryzhii,Michitaka Suzuki,Mitsuyoshi Nakao,Satoshi Waguri,Hideaki Morishita,Masaaki Komatsu

International journal of molecular sciences 26: PubMed40004139

2025

Proteomics and Expression of HIF2α/BNIP3L Signaling in Yak Brains at Different Altitudes.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Yan Cui,Sijiu Yu,Junfeng He,Yangyang Pan,Meng Wang,Jialing Che

Journal of experimental & clinical cancer research : CR 43:273 PubMed39350223

2024

Targeting DNM1L/DRP1-FIS1 axis inhibits high-grade glioma progression by impeding mitochondrial respiratory cristae remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Li,Jingjing Tie,Yuze Sun,Chengrong Gong,Shizhou Deng,Xiyu Chen,Shujiao Li,Yaoliang Wang,Zhenhua Wang,Feifei Wu,Hui Liu,Yousheng Wu,Guopeng Zhang,Qingdong Guo,Yanling Yang,Yayun Wang

Cell death & disease 11:251 PubMed32312970

2020

Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Duan,Lei Kuang,Xinming Xiang,Jie Zhang,Yu Zhu,Yue Wu,Qingguang Yan,Liangming Liu,Tao Li

View all publications

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Product promise

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