Rabbit Polyclonal BNIP3L/NIX antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BNIP3L aa 50-200.
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- Cell death & disease 11:2512020Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenyang Duan et. al.PubMed 32312970
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BNIP3L aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link O60238
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.
Alternative names
BNIP3A, BNIP3H, NIX, BNIP3L, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L
Recommended products
Rabbit Polyclonal BNIP3L/NIX antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BNIP3L aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BNIP3L aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link O60238
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-BNIP3L/NIX antibody (ab155010)
12% SDS PAGE
All lanes: Western blot - Anti-BNIP3L/NIX antibody (ab155010) at 1/1000 dilution
All lanes: Molt4 whole cell lysate at 30 µg
Predicted band size: 24 kDa
Western blot - Anti-BNIP3L/NIX antibody (ab155010)
12% SDS PAGE
All lanes: Western blot - Anti-BNIP3L/NIX antibody (ab155010) at 1/500 dilution
All lanes: mouse liver whole cell lysate at 50 µg
Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody (ab155010)
Immunohistochemical analysis of paraffin-embedded Human colon cancer labeling BNIP3L/NIX with ab155010 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody (ab155010)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling BNIP3L/NIX with ab155010 antibody at 1/200 dilution. Bottom figure: staining merged with DNA probe (Hoechst 33342).
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