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Rabbit Recombinant Monoclonal BNIP3L/NIX antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human BNIP3L conjugated to Keyhole Limpet Haemocyanin.

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Images

Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human BNIP3L conjugated to Keyhole Limpet Haemocyanin. Database link O60238

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Expected
Tested
Rat
Tested
Expected

Tested
Tested

Species

Rat

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.

Targets

Bnip3l

Alternative names

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L, BNIP3H, NIX, BNIP3A, BNIP3L

Recommended products

Rabbit Recombinant Monoclonal BNIP3L/NIX antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human BNIP3L conjugated to Keyhole Limpet Haemocyanin.

Alternative names

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L, BNIP3H, NIX, BNIP3A, BNIP3L

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human BNIP3L conjugated to Keyhole Limpet Haemocyanin. Database link O60238
Carrier free

Yes

Clone number

HL1203

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (ab313835), expandable thumbnail

    Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (ab313835)

    All lanes: Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (ab313835) at 1/1000 dilution

    All lanes: Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (ab313835), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (ab313835)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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