AB251494

Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal BNIP3L/NIX phospho S81 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 1 publication.

View Alternative Names

Nix, Bnip3l, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, NIP3-like protein X, NIP3L

2 Images

Supplier Data

Western blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (AB251494)

This data was developed using ab208190, the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in the literature (PMID : 10467396).

The plasmids were kindly provided by our collaborator Dr. Zhong Chen, Zhejiang University.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] (<a href='/en-us/products/primary-antibodies/bnip3l-nix-phospho-s81-antibody-epr19826-ab208190'>ab208190</a>) at 1/5000 dilution

Lane 1:

HEK-293 (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate at 10 µg

Lane 2:

HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, whole cell lysate at 10 µg

Lane 3:

HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, lysate then treated with alkaline phosphatase for 1 hour, whole cell lysate at 10 µg

Lane 4:

HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX S81A mutant expression vector, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 40 kDa,80 kDa

true

Supplier Data

Dot Blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (AB251494)

This data was developed using ab208190, the same antibody clone in a different buffer formulation.

Dot blot analysis of BNIP3L/NIX (phospho S81) labeled with ab208190 at 1/1000 dilution.

Lane 1 : BNIP3L/NIX (phospho S81) peptide;
Lane 2 : BNIP3L/NIX non-phospho peptide;

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Unconjugated

Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19826

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251494 is the carrier-free version of ab208190.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP : in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). May function as a tumor suppressor (By similarity).

See full target information Bnip3l pS81

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1180 PubMed40775066

2025

Regulation of cortical neurogenesis by MED13L via transcriptional priming and its implications for MED13L syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Li,Yu-Zhu Hao,Jia Cui,Xiao-Xiao Cao,Nan Wu,Ji Ma,Shuang-Yi Xie,Yi-Hsuan Pan,Xue-Lian He,Yu-Lan Zhao,Xiao-Bing Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free

Western blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (AB251494)

Dot Blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] - BSA and Azide free (AB251494)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise