AB243440

Anti-BNP antibody [EPR22339-65]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal BNP antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, Nppb, BNP(1-28), BNP(1-29), BNP(1-30), BNP(1-32), BNP(2-31), BNP(3-29), BNP(3-30), BNP(3-32), BNP(4-27), BNP(4-29), BNP(4-30), BNP(4-31), BNP(4-32), BNP(5-29), BNP(5-31), BNP(5-32), Brain natriuretic peptide 32, Brain type natriuretic peptide, Gamma-brain natriuretic peptide, NPPB, Natriuretic peptide B, Natriuretic peptide precursor B, Natriuretic peptides B, Natriuretic protein, natriuretic peptide, brain type

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

Negative tissue : no staining on rat liver (PMID : 15576492).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

Negative tissue : no staining on mouse liver (PMID : 15576492).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

Immunohistochemical analysis of paraffin-embedded rat heart tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the atrium (PMID : 15576492). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the atrium (PMID : 15576492). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Carrier free

Anti-BNP antibody [EPR22339-65] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22339-65

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Brain natriuretic peptide 45. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 8182124). May also function as a paracrine antifibrotic factor in the heart (PubMed : 10737768). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed : 8182124). Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis (By similarity).

See full target information Nppb

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

World journal of cardiology 17:104832 PubMed40575431

2025

Hydrogen alleviates right ventricular hypertrophy by inhibiting ferroptosis restoration of the Nrf2/HO-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Cai Bai,Hong-Xiao Yang,Cheng-Chuang Zhan,Lu-Qi Zhao,Jia-Ren Liu,Wei Yang

Physiological reports 12:e16022 PubMed38924383

2024

Overexpression of macrophage migration inhibitory factor protects against pressure overload-induced cardiac hypertrophy through regulating the miR-29b-3p/HBP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wen,Wei Chen,Cunjun Zhu,Jie Li,Juan Zhou,Minxia Zhang,Wenqiang Zhang,Qiang Xue

International heart journal 65:475-486 PubMed38825493

2024

Aspirin and Celecoxib Regulate Notch1/Hes1 Pathway to Prevent Pressure Overload-Induced Myocardial Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Minghui Wei,Ziyu Lu,Haifeng Zhang,Xiaomei Fan,Xin Zhang,Bihui Jiang,Jianying Li,Mingming Xue

Journal of healthcare engineering 2021:3843830 PubMed34956570

2021

Spironolactone Inhibits Cardiomyocyte Hypertrophy by Regulating the Ca/Calcineurin/p-NFATc3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Wenting Zhang,Jingtao Na,Yanping Huo,Yacheng Wang,Ketong Liu

Journal of healthcare engineering 2021:3433615 PubMed34900182

2021

Astragaloside IV Reduces OxLDL-Induced BNP Overexpression by Regulating HDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Zhang,Xin Wang,Jing Li,Mingyuan Xu,Xiaolu Ren,Huiying Liu,Lu Xu,Jun Shao

Experimental and therapeutic medicine 20:92 PubMed32973941

2020

lncRNA ZEB2-AS1 stimulates cardiac hypertrophy by downregulating PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Cheng,Lingyun Liu,Qingguo Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BNP antibody [EPR22339-65]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (AB243440)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise