AB3426

Anti-BOB1 antibody

Name: Anti-BOB1 antibody (ab3426) | Abcam
Brand: Abcam Limited
SKU: AB3426

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(2 Publications)

Goat Polyclonal BOB1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human POU2AF1 aa 200 to C-terminus.

View Alternative Names

BOB1, OBF1, POU2AF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1

1 Images

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Western blot - Anti-BOB1 antibody (AB3426)

ab3426 at a 1μg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 μg total protein per lane, 1 hour incubation) by Western blot (ECL). ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL).

All lanes:

Western blot - Anti-BOB1 antibody (ab3426)

Predicted band size: 27 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human POU2AF1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16633

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Detects a band of approximately 30 kDa in Mouse Spleen lysate.</p>" } } }

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Product details

Principal Names – POU2AF1; BOB1; OBF1; OCAB; OBF-1; POU domain, class 2, associating factor 1. Official Gene Symbol - POU2AF1. GenBank Accession Number – NP_006226. LocusLink ID - 5450 (human); 18985 (mouse). Gene Ontology terms - transcription cofactor activity; transcription co-activator activity; transcription activating factor; humoral immune response; transcription from Pol II promoter.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Constituents: 15% Sodium chloride, 3% Triton X-100, 3% Sodium deoxycholate, 0.3% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed : 7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed : 7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed : 7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed : 7623806, PubMed : 7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).

See full target information POU2AF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 19:442-452 PubMed29662169

2018

Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew V Russo,Lawrence L Latour,Dorian B McGavern

The American journal of pathology 181:2003-17 PubMed23063659

2012

Loss of semaphorin-neuropilin-1 signaling causes dysmorphic vascularization reminiscent of alveolar capillary dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen Joza,Jinxia Wang,Emily Fox,Valerie Hillman,Cameron Ackerley,Martin Post

View all publications

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Product promise

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