Anti-BOB1 antibody [EP3300]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (AB76010)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling BOB1 with unpurified ab76010 at 1/40 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] (AB76010)

ab76010 at 1/100 dilution staining BOB1 in human tonsil by immunohistochemistry using paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-BOB1 antibody [EP3300] (AB76010)

All lanes:

Western blot - Anti-BOB1 antibody [EP3300] (ab76010) at 1/30000 dilution

All lanes:

Ramos cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 35 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-BOB1 antibody [EP3300] (AB76010)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.