Anti-BOB1 antibody [EPR17685] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392)

This data was developed using ab203110, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Non-Hodgkin lymphoma of B- lineage tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weak cytoplasmic staining on Human non-hodgkin lymphoma of B- lineage tissue is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Reference : Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2) : 501–507.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392)

This data was developed using ab203110, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weakly cytoplasmic staining on lymphocyte of Human colon lineage tissue is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Reference : Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2) : 501–507.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392)

This data was developed using ab203110, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human cerebral cortex tissue (BOB1 Negative tissue) is observed.

Negative control : Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Reference : Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2) : 501–507.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392)

This data was developed using ab203110, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The MW profile observed is consistent with what has been described in the literature (PMID : 12857960).

All lanes:

Western blot - Anti-BOB1 antibody [EPR17685] (<a href='/en-us/products/primary-antibodies/bob1-antibody-epr17685-ab203110'>ab203110</a>) at 1/10000 dilution

All lanes:

RAMOS (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 35 kDa

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392)

This data was developed using ab203110, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The MW profile observed is consistent with what has been described in the literature (PMID : 12857960).

All lanes:

Western blot - Anti-BOB1 antibody [EPR17685] (<a href='/en-us/products/primary-antibodies/bob1-antibody-epr17685-ab203110'>ab203110</a>) at 1/5000 dilution

All lanes:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 35 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392)

This data was developed using ab203110, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The MW profile observed is consistent with what has been described in the literature (PMID : 12857960).

All lanes:

Western blot - Anti-BOB1 antibody [EPR17685] (<a href='/en-us/products/primary-antibodies/bob1-antibody-epr17685-ab203110'>ab203110</a>) at 1/200 dilution

Lane 1:

Human lymph node whole cell lysate at 10 µg

Lane 2:

Human bone marrow whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 35 kDa

false

Exposure time: 3min