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Rabbit Recombinant Monoclonal BOB1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392), expandable thumbnail
  • Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392), expandable thumbnail
  • Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (AB251392), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).

Alternative names

BOB1, OBF1, POU2AF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1

Recommended products

Rabbit Recombinant Monoclonal BOB1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR17685
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251392 is the carrier-free version of Anti-BOB1 antibody [EPR17685] ab203110.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392), expandable thumbnail

    Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)

    This data was developed using Anti-BOB1 antibody [EPR17685] ab203110, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The MW profile observed is consistent with what has been described in the literature (PMID: 12857960).

    All lanes: Western blot - Anti-BOB1 antibody [EPR17685] (Anti-BOB1 antibody [EPR17685] ab203110) at 1/10000 dilution

    All lanes: RAMOS (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 27 kDa

    Observed band size: 35 kDa

    Exposure time: 5s

  • Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392), expandable thumbnail

    Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)

    This data was developed using Anti-BOB1 antibody [EPR17685] ab203110, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The MW profile observed is consistent with what has been described in the literature (PMID: 12857960).

    All lanes: Western blot - Anti-BOB1 antibody [EPR17685] (Anti-BOB1 antibody [EPR17685] ab203110) at 1/5000 dilution

    All lanes: Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 27 kDa

    Observed band size: 35 kDa

    Exposure time: 3min

  • Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392), expandable thumbnail

    Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)

    This data was developed using Anti-BOB1 antibody [EPR17685] ab203110, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The MW profile observed is consistent with what has been described in the literature (PMID: 12857960).

    All lanes: Western blot - Anti-BOB1 antibody [EPR17685] (Anti-BOB1 antibody [EPR17685] ab203110) at 1/200 dilution

    Lane 1: Human lymph node whole cell lysate at 10 µg

    Lane 2: Human bone marrow whole cell lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 27 kDa

    Observed band size: 35 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)

    This data was developed using Anti-BOB1 antibody [EPR17685] ab203110, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Non-Hodgkin lymphoma of B- lineage tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weak cytoplasmic staining on Human non-hodgkin lymphoma of B- lineage tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP). Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)

    This data was developed using Anti-BOB1 antibody [EPR17685] ab203110, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weakly cytoplasmic staining on lymphocyte of Human colon lineage tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP). Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)

    This data was developed using Anti-BOB1 antibody [EPR17685] ab203110, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human cerebral cortex tissue (BOB1 Negative tissue) is observed. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP). Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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