AB92315

Anti-BOB1 antibody [EPR3299]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal BOB1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

BOB1, OBF1, POU2AF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1

1 Images

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Western blot - Anti-BOB1 antibody [EPR3299] (AB92315)

All lanes:

Western blot - Anti-BOB1 antibody [EPR3299] (ab92315) at 1/2000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

Daudi cell lysate at 10 µg

Lane 3:

NAMALWA cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3299

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed : 7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed : 7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed : 7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed : 7623806, PubMed : 7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).

See full target information POU2AF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:474 PubMed39774694

2025

Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Bai,Jinxin Xu,Wenbin Gu,Danyang Wang,Ying Cui,Weidong Rong,Xiaoan Du,Xiaoxia Li,Cuicui Xia,Qingqing Gan,Guantao He,Huahui Guo,Jinfeng Deng,Yuqiong Wu,Ray-Whay Chiu Yen,Srinivasan Yegnasubramanian,Scott B Rothbart,Cheng Luo,Linping Wu,Jinsong Liu,Stephen B Baylin,Xiangqian Kong

Molecular cell 80:845-861.e10 PubMed33232656

2020

Unique Immune Cell Coactivators Specify Locus Control Region Function and Cell Stage.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Shuen Chu,Johannes C Hellmuth,Rajat Singh,Hsia-Yuan Ying,Lucy Skrabanek,Matthew R Teater,Ashley S Doane,Olivier Elemento,Ari M Melnick,Robert G Roeder

View all publications

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