AB157202

Anti-BOG antibody [EPR9905]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal BOG antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BOG, RBBP10, RBBP9, Serine hydrolase RBBP9, B5T-overexpressed gene protein, Retinoblastoma-binding protein 10, Retinoblastoma-binding protein 9, Protein BOG, RBBP-10, RBBP-9

4 Images

Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] (AB157202)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] (AB157202)

Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling BOG (red), using ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] (AB157202)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] (AB157202)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue labeling BOG using ab157202 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] (AB157202)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] (AB157202)

Immunofluorescence analysis of MCF7 cells labeling BOG using ab157202 at a 1/250 dilution.

Unknown

Western blot - Anti-BOG antibody [EPR9905] (AB157202)

All lanes:

Western blot - Anti-BOG antibody [EPR9905] (ab157202) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9905

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Serine hydrolase whose substrates have not been identified yet (PubMed : 19329999, PubMed : 20080647). May negatively regulate basal or autocrine TGF-beta signaling by suppressing SMAD2-SMAD3 phosphorylation (PubMed : 20080647). May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta through interaction with RB1 and the subsequent displacement of E2F1 (PubMed : 9697699).

See full target information RBBP9

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 42:45 PubMed36759880

2023

Procoxacin bidirectionally inhibits osteoblastic and osteoclastic activity in bone and suppresses bone metastasis of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Depei Kong,Chen Ye,Chenxi Zhang,Xiaochen Sun,Fubo Wang,Rui Chen,Guangan Xiao,Shipeng He,Jianrong Xu,Xiwu Rao,Jianzhong Ai,Xu Gao,Hong Li,Li Su

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BOG antibody [EPR9905]

Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] (AB157202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] (AB157202)

Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] (AB157202)

Western blot - Anti-BOG antibody [EPR9905] (AB157202)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise