Rabbit Recombinant Monoclonal BOG antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307), expandable thumbnail](https://content.abcam.com/products/images/bog-antibody-epr9905-bsa-and-azide-free-ab249307--western-blot-img106970.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307), expandable thumbnail](https://content.abcam.com/products/images/bog-antibody-epr9905-bsa-and-azide-free-ab249307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129067.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)")
![Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307), expandable thumbnail](https://content.abcam.com/products/images/bog-antibody-epr9905-bsa-and-azide-free-ab249307--immunocytochemistry-immunofluorescence-img24078.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)")
![Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307), expandable thumbnail](https://content.abcam.com/products/images/bog-antibody-epr9905-bsa-and-azide-free-ab249307--flow-cytometry-intracellular-img88972.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Rat | Predicted | Predicted | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Serine hydrolase whose substrates have not been identified yet (PubMed:19329999, PubMed:20080647). May negatively regulate basal or autocrine TGF-beta signaling by suppressing SMAD2-SMAD3 phosphorylation (PubMed:20080647). May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta through interaction with RB1 and the subsequent displacement of E2F1 (PubMed:9697699).
Alternative names
BOG, RBBP10, RBBP9, Serine hydrolase RBBP9, B5T-overexpressed gene protein, Retinoblastoma-binding protein 10, Retinoblastoma-binding protein 9, Protein BOG, RBBP-10, RBBP-9
Recommended products
Rabbit Recombinant Monoclonal BOG antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9905
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249307 is the carrier-free version of Anti-BOG antibody [EPR9905] ab157202.
Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307), expandable thumbnail](https://content.abcam.com/products/images/bog-antibody-epr9905-bsa-and-azide-free-ab249307--western-blot-img106970.jpg "Western blot - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307)")
Western blot - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307)
This data was developed using Anti-BOG antibody [EPR9905] ab157202, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-BOG antibody [EPR9905] (Anti-BOG antibody [EPR9905] ab157202) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: MCF-7 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Predicted band size: 21 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307), expandable thumbnail](https://content.abcam.com/products/images/bog-antibody-epr9905-bsa-and-azide-free-ab249307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129067.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307)
This data was developed using Anti-BOG antibody [EPR9905] ab157202, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling BOG using Anti-BOG antibody [EPR9905] ab157202 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307), expandable thumbnail](https://content.abcam.com/products/images/bog-antibody-epr9905-bsa-and-azide-free-ab249307--immunocytochemistry-immunofluorescence-img24078.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307)")
Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307)
This data was developed using Anti-BOG antibody [EPR9905] ab157202, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of MCF7 cells, labeling BOG using Anti-BOG antibody [EPR9905] ab157202 at a 1/250 dilution.
![Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307), expandable thumbnail](https://content.abcam.com/products/images/bog-antibody-epr9905-bsa-and-azide-free-ab249307--flow-cytometry-intracellular-img88972.jpg "Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307)")
Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] - BSA and Azide free (ab249307)
This data was developed using Anti-BOG antibody [EPR9905] ab157202, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling BOG (red), using Anti-BOG antibody [EPR9905] ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)
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