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Rabbit Recombinant Monoclonal BOK antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

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Images

Western blot - Anti-Bok antibody [BOK-R1-5-1] (AB233072), expandable thumbnail
  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (AB233072), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (AB233072), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (AB233072), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Isoform 1. Apoptosis regulator that functions through different apoptotic signaling pathways (PubMed:15102863, PubMed:20673843, PubMed:27076518). Plays a roles as pro-apoptotic protein that positively regulates intrinsic apoptotic process in a BAX- and BAK1-dependent manner or in a BAX- and BAK1-independent manner (PubMed:15102863, PubMed:27076518). In response to endoplasmic reticulum stress promotes mitochondrial apoptosis through downstream BAX/BAK1 activation and positive regulation of PERK-mediated unfolded protein response (By similarity). Activates apoptosis independently of heterodimerization with survival-promoting BCL2 and BCL2L1 through induction of mitochondrial outer membrane permeabilization, in a BAX- and BAK1-independent manner, in response to inhibition of ERAD-proteasome degradation system, resulting in cytochrome c release (PubMed:27076518). In response to DNA damage, mediates intrinsic apoptotic process in a TP53-dependent manner (PubMed:15102863). Plays a role in granulosa cell apoptosis by CASP3 activation (PubMed:20673843). Plays a roles as anti-apoptotic protein during neuronal apoptotic process, by negatively regulating poly ADP-ribose polymerase-dependent cell death through regulation of neuronal calcium homeostasis and mitochondrial bioenergetics in response to NMDA excitation (By similarity). In addition to its role in apoptosis, may regulate trophoblast cell proliferation during the early stages of placental development, by acting on G1/S transition through regulation of CCNE1 expression (PubMed:19942931). May also play a role as an inducer of autophagy by disrupting interaction between MCL1 and BECN1 (PubMed:24113155). Isoform 2. Pro-apoptotic molecule exerting its function through the mitochondrial pathway.

Alternative names

BCL2L9, BOK, Bcl-2-related ovarian killer protein, hBOK, Bcl-2-like protein 9, Bcl2-L-9

Recommended products

Rabbit Recombinant Monoclonal BOK antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
BOK-R1-5-1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

BCL2-related ovarian killer (Bok) is a pro-apoptotic member of the Bcl-2 protein family contributing to apoptosis regulation. Bok sometimes referred to by its weight as approximately 23-25 kDa facilitates programmed cell death through mitochondrial pathways. Researchers have identified its expression primarily in reproductive tissues like ovaries and testis with some presence in the liver. Bok interacts with other Bcl-2 family proteins which act as apoptosis regulators creating a balance between cell survival and death.

Biological function summary

Bok plays an important role in the intrinsic apoptosis pathway by modulating mitochondrial outer membrane permeabilization. Bok forms protein complexes with other Bcl-2 family members like Bax and Bak contributing to cytochrome c release. This release is important for the activation of caspases leading to cellular apoptosis. Bok also exercises a regulatory role on calcium homeostasis within cells although its exact mechanisms remain under study.

Pathways

Bok integrates into the mitochondrial apoptosis pathway and the endoplasmic reticulum stress response influencing cellular fate decisions. Within these pathways Bok interacts with proteins such as Bax Bak and Bcl-2 balancing pro-apoptotic and anti-apoptotic signals. Bok's ability to influence these pathways demonstrates its importance in maintaining cellular homeostasis and response to stress signals.

Associated diseases and disorders

Bok has been associated with cancer and neurodegenerative diseases. Its role in promoting apoptosis links it to conditions where apoptosis is dysregulated such as tumorigenesis where reduced Bok activity could contribute to cancer progression. In neurodegenerative disorders like Alzheimer's Bok's interaction with proteins like Bax may exacerbate neuronal loss through increased apoptosis emphasizing the need for understanding Bok in developing therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072), expandable thumbnail

    Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.

    All lanes: Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution

    Lane 1: Wild-type MEF (Mouse embryonic fibroblast cell line) cell lysate at 20 µg

    Lane 2: Bok knockout MEF cell lysate at 20 µg

    Lane 3: Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

    Lane 4: Bok knockout HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa

    Exposure time: 70s

  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072), expandable thumbnail

    Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-3 and 8: 70 seconds; Lanes 4-5: 125 seconds; Lanes 6-7: 37 seconds; Lane 9: 3 minutes.

    The specific band for BOK is observed at 22 kDa; the other bands on the blots are non-specific.

    Lanes 1 - 3: Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution

    Lanes 4 - 9: Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Human fetal liver at 20 µg

    Lane 3: Human fetal kidney at 20 µg

    Lane 4: Mouse brain at 10 µg

    Lane 5: Mouse liver at 10 µg

    Lane 6: Rat brain at 10 µg

    Lane 7: Rat spleen at 10 µg

    Lane 8: C6 (rat glial tumor cell) whole cell lysate at 10 µg

    Lane 9: Human fetal brain at 10 µg

    Secondary

    Lanes 1 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lane 9: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution

    Predicted band size: 23 kDa

  • Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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