JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB233700

Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal BOK antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

BCL2L9, BOK, Bcl-2-related ovarian killer protein, hBOK, Bcl-2-like protein 9, Bcl2-L-9

3 Images
Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free (AB233700)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free (AB233700)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233072).

Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free (AB233700)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free (AB233700)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233072).

Western blot - Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free (AB233700)
  • WB

Supplier Data

Western blot - Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free (AB233700)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233072).

All lanes:

Western blot - Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free (ab233700) at 1/5000 dilution

Lane 1:

Wild-type MEF cell lysate at 20 µg

Lane 2:

Bok knockout MEF cell lysate at 20 µg

Lane 3:

Wild-type HepG2 cell lysate at 20 µg

Lane 4:

Bok knockout HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 23 kDa

false

Exposure time: 70s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BOK-R1-5-1

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

ab233700 is the carrier-free version of ab233072.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BCL2-related ovarian killer (Bok) is a pro-apoptotic member of the Bcl-2 protein family contributing to apoptosis regulation. Bok sometimes referred to by its weight as approximately 23-25 kDa facilitates programmed cell death through mitochondrial pathways. Researchers have identified its expression primarily in reproductive tissues like ovaries and testis with some presence in the liver. Bok interacts with other Bcl-2 family proteins which act as apoptosis regulators creating a balance between cell survival and death.
Biological function summary

Bok plays an important role in the intrinsic apoptosis pathway by modulating mitochondrial outer membrane permeabilization. Bok forms protein complexes with other Bcl-2 family members like Bax and Bak contributing to cytochrome c release. This release is important for the activation of caspases leading to cellular apoptosis. Bok also exercises a regulatory role on calcium homeostasis within cells although its exact mechanisms remain under study.

Pathways

Bok integrates into the mitochondrial apoptosis pathway and the endoplasmic reticulum stress response influencing cellular fate decisions. Within these pathways Bok interacts with proteins such as Bax Bak and Bcl-2 balancing pro-apoptotic and anti-apoptotic signals. Bok's ability to influence these pathways demonstrates its importance in maintaining cellular homeostasis and response to stress signals.

Bok has been associated with cancer and neurodegenerative diseases. Its role in promoting apoptosis links it to conditions where apoptosis is dysregulated such as tumorigenesis where reduced Bok activity could contribute to cancer progression. In neurodegenerative disorders like Alzheimer's Bok's interaction with proteins like Bax may exacerbate neuronal loss through increased apoptosis emphasizing the need for understanding Bok in developing therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Isoform 1. Apoptosis regulator that functions through different apoptotic signaling pathways (PubMed : 15102863, PubMed : 20673843, PubMed : 27076518). Plays a roles as pro-apoptotic protein that positively regulates intrinsic apoptotic process in a BAX- and BAK1-dependent manner or in a BAX- and BAK1-independent manner (PubMed : 15102863, PubMed : 27076518). In response to endoplasmic reticulum stress promotes mitochondrial apoptosis through downstream BAX/BAK1 activation and positive regulation of PERK-mediated unfolded protein response (By similarity). Activates apoptosis independently of heterodimerization with survival-promoting BCL2 and BCL2L1 through induction of mitochondrial outer membrane permeabilization, in a BAX- and BAK1-independent manner, in response to inhibition of ERAD-proteasome degradation system, resulting in cytochrome c release (PubMed : 27076518). In response to DNA damage, mediates intrinsic apoptotic process in a TP53-dependent manner (PubMed : 15102863). Plays a role in granulosa cell apoptosis by CASP3 activation (PubMed : 20673843). Plays a roles as anti-apoptotic protein during neuronal apoptotic process, by negatively regulating poly ADP-ribose polymerase-dependent cell death through regulation of neuronal calcium homeostasis and mitochondrial bioenergetics in response to NMDA excitation (By similarity). In addition to its role in apoptosis, may regulate trophoblast cell proliferation during the early stages of placental development, by acting on G1/S transition through regulation of CCNE1 expression (PubMed : 19942931). May also play a role as an inducer of autophagy by disrupting interaction between MCL1 and BECN1 (PubMed : 24113155).. Isoform 2. Pro-apoptotic molecule exerting its function through the mitochondrial pathway.
See full target information BOK
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroreport 36:350-363 PubMed40203233

2025

Long noncoding RNA MALAT1 promotes angiogenesis through the caveolin-1/VEGF pathway after cerebral ischemic injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lin,Qiongyi Pang,Yuanxi Shi,Xiang Chen,Fengxia Tu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB305476

PE Anti-Bok antibody [BOK-R1-5-1]

RabMAb|Recombinant

  • 0
  • 0
Primary Antibodies

AB305477

APC Anti-Bok antibody [BOK-R1-5-1]

RabMAb|Recombinant

  • 0
  • 0
Primary Antibodies

AB186745

Anti-Bok antibody [EPR15331]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Bok antibody [EPR15331] (AB186745)

  • 0
  • 0
Primary Antibodies

AB232610

Anti-Bok antibody [EPR15331] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Bok antibody [EPR15331] - BSA and Azide free (AB232610)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109199

Anti-p21 antibody [EPR3993]

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-p21 antibody [EPR3993] (AB109199)

  • 4
  • 12
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com