Rabbit Recombinant Monoclonal Bone Sialoprotein antibody. Carrier free. Suitable for IP, IHC-Fr, IHC-P and reacts with Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (AB272320), expandable thumbnail](https://content.abcam.com/products/images/bone-sialoprotein-antibody-epr23367-50-bsa-and-azide-free-ab272320--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100587.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (AB272320)")
![Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (AB272320), expandable thumbnail](https://content.abcam.com/products/images/bone-sialoprotein-antibody-epr23367-50-bsa-and-azide-free-ab272320--immunoprecipitation-img11642.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (AB272320)")
![Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (AB272320), expandable thumbnail](https://content.abcam.com/products/images/bone-sialoprotein-antibody-epr23367-50-bsa-and-azide-free-ab272320--immunohistochemistry-frozen-sections-img5069.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (AB272320)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes adhesion and migration of various cells via the alpha-V/beta-3 integrin receptor (ITGAV:ITGB3).
Alternative names
Integrin-binding sialoprotein, Bone sialoprotein 2, Bone sialoprotein II, Cell-binding sialoprotein, BSP II, Ibsp
Recommended products
Rabbit Recombinant Monoclonal Bone Sialoprotein antibody. Carrier free. Suitable for IP, IHC-Fr, IHC-P and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23367-50
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab272320 is the carrier-free version of Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bone Sialoprotein (BSP) is a non-collagenous glycoprotein of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family with a mass around 70-80 kDa. Its expression mainly takes place in mineralized tissues like bone and dentin highlighting its importance in skeletal biology. BSP has a strong affinity for calcium and hydroxyapatite allowing it to play binding and structural roles in bone matrix formation.
- Biological function summary
The protein influences the nucleation of hydroxyapatite crystals and helps regulate mineralization processes. It associates with collagenous and non-collagenous components in the bone and contributes to tissue integrity and repair. BSP functions as part of a complex matrix that orchestrates cell adhesion and signal transduction impacting osteoblast and osteoclast activity.
- Pathways
Bone Sialoprotein interacts within the extracellular matrix organization pathway and the integrin signaling pathway. In the extracellular matrix pathway BSP plays a role together with proteins like osteopontin also from the SIBLING family to influence cellular adhesion. Through integrin signaling BSP modulates cell-surface interactions particularly affecting the behavior of osteoclasts and osteoblasts and their capacity to maintain bone homeostasis.
- Associated diseases and disorders
Bone Sialoprotein associates with pathological conditions such as osteosarcoma and osteoporosis. In osteosarcoma BSP overexpression correlates with aggressive tumor growth potentially linking it with the tumor protein osteopontin suggesting a marker for malignancy. In osteoporosis dysregulation of BSP expression may affect bone density contributing to weakened bone structure alongside changes in other bone matrix proteins.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320), expandable thumbnail](https://content.abcam.com/products/images/bone-sialoprotein-antibody-epr23367-50-bsa-and-azide-free-ab272320--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100587.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
Immunohistochemical analysis of paraffin-embedded Mouse E14.5 tissue labeling Bone Sialoprotein with Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on bones of mouse E14.5 (PMID: 24927920). The section was incubated with Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457).
![Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320), expandable thumbnail](https://content.abcam.com/products/images/bone-sialoprotein-antibody-epr23367-50-bsa-and-azide-free-ab272320--immunoprecipitation-img11642.jpg "Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)")
Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
Bone Sialoprotein was immunoprecipitated from 0.35 mg Mouse fetal bone tissue lysate using Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse fetal bone tissue lysate 10ug
Lane 2: Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457 IP in Mouse fetal bone tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457 in Mouse fetal bone tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 sseconds.
The molecular weight observed is consistent with literature (PMID: 14647232)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457).
All lanes: Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] (Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457)
Predicted band size: 35 kDa
Observed band size: 80 kDa
![Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320), expandable thumbnail](https://content.abcam.com/products/images/bone-sialoprotein-antibody-epr23367-50-bsa-and-azide-free-ab272320--immunohistochemistry-frozen-sections-img5069.jpg "Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)")
Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-1-- permeabilized mouse E14.5 embryo tissue labeling Bone Sialoprotein with Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse embryonic bone. The nuclear counter stain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Secondary antibody is b150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bone Sialoprotein antibody [EPR23367-50] ab270457).
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