AB86653

Anti-BOP1 antibody

Name: Anti-BOP1 antibody (ab86653) | Abcam
Brand: Abcam Limited
SKU: AB86653

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(1 Publication)

Rabbit Polyclonal BOP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BOP1 aa 300-350.

View Alternative Names

KIAA0124, BOP1, Ribosome biogenesis protein BOP1, Block of proliferation 1 protein

2 Images

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Immunoprecipitation - Anti-BOP1 antibody (AB86653)

Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 μg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with exposure time of 90 seconds

All lanes:

Immunoprecipitation - Anti-BOP1 antibody (ab86653)

Predicted band size: 84 kDa

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Unknown

Western blot - Anti-BOP1 antibody (AB86653)

Detection : Chemiluminescence with exposure time of 90 seconds

All lanes:

Western blot - Anti-BOP1 antibody (ab86653) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 84 kDa

Observed band size: 100 kDa,110 kDa,120 kDa,65 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human BOP1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q14137

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.

See full target information BOP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology : PubMed32805281

2020

The Long Noncoding RNA CCAT2 induces chromosomal instability through BOP1 - AURKB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Baoqing Chen,Mihnea P Dragomir,Linda Fabris,Recep Bayraktar,Erik Knutsen,Xu Liu,Changyan Tang,Yongfeng Li,Tadanobu Shimura,Tina Catela Ivkovic,Mireia Cruz De Los Santos,Simone Anfossi,Masayoshi Shimizu,Maitri Y Shah,Hui Ling,Peng Shen,Asha S Multani,Barbara Pardini,Jared K Burks,Hiroyuki Katayama,Lucas C Reineke,Longfei Huo,Muddassir Syed,Shumei Song,Manuela Ferracin,Eiji Oki,Bastian Fromm,Cristina Ivan,Krithika Bhuvaneshwar,Yuriy Gusev,Koshi Mimori,David Menter,Subrata Sen,Takatoshi Matsuyama,Hiroyuki Uetake,Catalin Vasilescu,Scott Kopetz,Jan Parker-Thornburg,Ayumu Taguchi,Samir M Hanash,Leonard Girnita,Ondrej Slaby,Ajay Goel,Gabriele Varani,Mihai Gagea,Chunlai Li,Jaffer A Ajani,George A Calin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BOP1 antibody

Immunoprecipitation - Anti-BOP1 antibody (AB86653)

Western blot - Anti-BOP1 antibody (AB86653)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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