AB182149

Anti-Bora antibody [EPR11610]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal BORA antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

C13orf34, BORA, Protein aurora borealis, HsBora

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Bora antibody [EPR11610] (AB182149)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bora antibody [EPR11610] (AB182149)

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling Bora with ab182149 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 diution.

Supplier Data

Immunoprecipitation - Anti-Bora antibody [EPR11610] (AB182149)

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab182149 at 1/40 dilution.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Bora antibody [EPR11610] (ab182149)

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-Bora antibody [EPR11610] (AB182149)

All lanes:

Western blot - Anti-Bora antibody [EPR11610] (ab182149) at 1/10000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-Bora antibody [EPR11610] (AB182149)

All lanes:

Western blot - Anti-Bora antibody [EPR11610] (ab182149) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

false

Carrier free

Anti-Bora antibody [EPR11610] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11610

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bora protein also known by its aliases Bora Rabbit or Bora Compliance plays a significant role in cell cycle regulation. It is a cofactor that primarily interacts with Aurora A kinase to promote activation. Bora protein weighs around 58 kilodaltons and is notably expressed in various tissues including those involved in cell division activities such as the reproductive organs and the skin.

Biological function summary

Bora protein's primary role involves cell cycle progression and the maintenance of genomic integrity. It forms part of a regulatory complex that ensures proper mitotic entry linking it closely with mitotic spindle formation and centrosome maturation processes. The Bora protein is pivotal in activating Aurora A kinase therefore ensuring accurate chromosome segregation during mitosis.

Pathways

The Bora protein is deeply integrated into cell cycle regulation and mitotic spindle checkpoint pathways. It functions as an upstream regulator within the Aurora A kinase pathway impacting downstream processes that involve key proteins such as Plk1 and Cdc25B. Through these interactions Bora protein helps monitor and coordinate entry into mitosis highlighting its centrality in mitotic timing and fidelity.

Abnormal expression or mutations in the Bora protein can lead to tumorigenesis and cancer progression. Specifically it is associated with disorders such as breast cancer and ovarian cancer. The dysregulation of Bora's interaction with Aurora A kinase and other proteins like p53 can contribute to aberrant cell cycle progression highlighting its involvement in the pathogenesis of cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the activation of AURKA at the onset of mitosis.

See full target information BORA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed39791720

2025

Induction of SUSD2 by STAT3 Activation Is Associated with Tumor Recurrence in HER2-Positive Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yisun Jeong,Hyungjoo Kim,Daeun You,Soo Youn Cho,Sun Young Yoon,Seok Won Kim,Seok Jin Nam,Jeong Eon Lee,Sangmin Kim

Journal of hematology & oncology 12:67 PubMed31253167

2019

Potential immune escape mechanisms underlying the distinct clinical outcome of immune checkpoint blockades in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Tian,Xiaoyang Zhai,Anqin Han,Hui Zhu,Jinming Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bora antibody [EPR11610]

Immunocytochemistry/ Immunofluorescence - Anti-Bora antibody [EPR11610] (AB182149)

Immunoprecipitation - Anti-Bora antibody [EPR11610] (AB182149)

Western blot - Anti-Bora antibody [EPR11610] (AB182149)

Western blot - Anti-Bora antibody [EPR11610] (AB182149)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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