JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250591

Anti-Bora antibody [EPR11610] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal BORA antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C13orf34, BORA, Protein aurora borealis, HsBora

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Bora antibody [EPR11610] - BSA and Azide free (AB250591)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bora antibody [EPR11610] - BSA and Azide free (AB250591)

This data was developed using ab182149, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling Bora with ab182149 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 diution.

Immunoprecipitation - Anti-Bora antibody [EPR11610] - BSA and Azide free (AB250591)
  • IP

Supplier Data

Immunoprecipitation - Anti-Bora antibody [EPR11610] - BSA and Azide free (AB250591)

This data was developed using ab182149, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab182149 at 1/40 dilution.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Bora antibody [EPR11610] (<a href='/en-us/products/primary-antibodies/bora-antibody-epr11610-ab182149'>ab182149</a>)

Predicted band size: 61 kDa

false

Western blot - Anti-Bora antibody [EPR11610] - BSA and Azide free (AB250591)
  • WB

Supplier Data

Western blot - Anti-Bora antibody [EPR11610] - BSA and Azide free (AB250591)

This data was developed using ab182149, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Bora antibody [EPR11610] (<a href='/en-us/products/primary-antibodies/bora-antibody-epr11610-ab182149'>ab182149</a>) at 1/10000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

false

Western blot - Anti-Bora antibody [EPR11610] - BSA and Azide free (AB250591)
  • WB

Supplier Data

Western blot - Anti-Bora antibody [EPR11610] - BSA and Azide free (AB250591)

This data was developed using ab182149, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Bora antibody [EPR11610] (<a href='/en-us/products/primary-antibodies/bora-antibody-epr11610-ab182149'>ab182149</a>) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11610

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

ab250591 is the carrier-free version of ab182149.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bora protein also known by its aliases Bora Rabbit or Bora Compliance plays a significant role in cell cycle regulation. It is a cofactor that primarily interacts with Aurora A kinase to promote activation. Bora protein weighs around 58 kilodaltons and is notably expressed in various tissues including those involved in cell division activities such as the reproductive organs and the skin.
Biological function summary

Bora protein's primary role involves cell cycle progression and the maintenance of genomic integrity. It forms part of a regulatory complex that ensures proper mitotic entry linking it closely with mitotic spindle formation and centrosome maturation processes. The Bora protein is pivotal in activating Aurora A kinase therefore ensuring accurate chromosome segregation during mitosis.

Pathways

The Bora protein is deeply integrated into cell cycle regulation and mitotic spindle checkpoint pathways. It functions as an upstream regulator within the Aurora A kinase pathway impacting downstream processes that involve key proteins such as Plk1 and Cdc25B. Through these interactions Bora protein helps monitor and coordinate entry into mitosis highlighting its centrality in mitotic timing and fidelity.

Abnormal expression or mutations in the Bora protein can lead to tumorigenesis and cancer progression. Specifically it is associated with disorders such as breast cancer and ovarian cancer. The dysregulation of Bora's interaction with Aurora A kinase and other proteins like p53 can contribute to aberrant cell cycle progression highlighting its involvement in the pathogenesis of cancer.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for the activation of AURKA at the onset of mitosis.
See full target information BORA
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB210528

Anti-ZIP Kinase antibody [EPR18809-86]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody [EPR18809-86] (AB210528)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB109118

Anti-PTP4A2/PRL2 antibody [EPR3800]

RabMAb|Recombinant

Western blot - Anti-PTP4A2/PRL2 antibody [EPR3800] (AB109118)

  • 0
  • 0
Primary Antibodies

AB75765

Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722]

RabMAb|Recombinant

Western blot - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (AB75765)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com