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AB308116

Anti-Bora antibody [RP23040151]

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Rabbit Recombinant Multiclonal BORA antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human BORA.

View Alternative Names

C13orf34, BORA, Protein aurora borealis, HsBora

1 Images
Western blot - Anti-Bora antibody [RP23040151] (AB308116)
  • WB

Supplier Data

Western blot - Anti-Bora antibody [RP23040151] (AB308116)

Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), HCT116 (Lane 4), SH-SY5Y (Lane 5), Mouse Heart (Lane 6). The blots were probed with ab308116 at 1-2 µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution. A ~ 52 kDa band corresponding to BORA was observed. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-Bora antibody [RP23040151] (ab308116) at 1 µg/mL

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

Jurkat cell lysate at 30 µg

Lane 4:

HCT 116 cell lysate at 30 µg

Lane 5:

SH-SY5Y cell lysate at 30 µg

Lane 6:

Mouse heart tissue lysate at 30 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2500 dilution

Observed band size: 53 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040151

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human BORA.

Q6PGQ7

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Reactivity data

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bora protein also known by its aliases Bora Rabbit or Bora Compliance plays a significant role in cell cycle regulation. It is a cofactor that primarily interacts with Aurora A kinase to promote activation. Bora protein weighs around 58 kilodaltons and is notably expressed in various tissues including those involved in cell division activities such as the reproductive organs and the skin.
Biological function summary

Bora protein's primary role involves cell cycle progression and the maintenance of genomic integrity. It forms part of a regulatory complex that ensures proper mitotic entry linking it closely with mitotic spindle formation and centrosome maturation processes. The Bora protein is pivotal in activating Aurora A kinase therefore ensuring accurate chromosome segregation during mitosis.

Pathways

The Bora protein is deeply integrated into cell cycle regulation and mitotic spindle checkpoint pathways. It functions as an upstream regulator within the Aurora A kinase pathway impacting downstream processes that involve key proteins such as Plk1 and Cdc25B. Through these interactions Bora protein helps monitor and coordinate entry into mitosis highlighting its centrality in mitotic timing and fidelity.

Abnormal expression or mutations in the Bora protein can lead to tumorigenesis and cancer progression. Specifically it is associated with disorders such as breast cancer and ovarian cancer. The dysregulation of Bora's interaction with Aurora A kinase and other proteins like p53 can contribute to aberrant cell cycle progression highlighting its involvement in the pathogenesis of cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the activation of AURKA at the onset of mitosis.
See full target information BORA
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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