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AB27158

Anti-Bordetella pertussis toxin antibody [20.6]

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(1 Publication)

Mouse Monoclonal TOX2 antibody. Carrier free. Suitable for ELISA, WB and reacts with Bordetella pertussis samples. Cited in 1 publication.

View Alternative Names

BP3784, ptxB, Pertussis toxin subunit 2, PTX S2, Islet-activating protein S2, IAP S2

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

20.6

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Bordetella pertussis

Applications

WB, ELISA

applications

Specificity

<p>Reacts with the intact toxin as well as the S2 subunit. Some reactivity towards the toxoid is also present.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bordetella pertussis": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/100000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS, 2.9% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bordetella pertussis toxin often called pertussis toxin or PTX functions mechanically as an exotoxin produced by Bordetella pertussis. This toxin has a molecular mass of around 105 kDa and comes from the bacterium responsible for whooping cough. It expresses itself in various host cells after the bacterium releases it into the respiratory tract during infection. The pertussis toxin consists of five subunits S1 through S5 which work together to impair host cellular functions by catalyzing the ADP-ribosylation of G-proteins.
Biological function summary

Pertussis toxin disrupts host immune responses and cellular signaling pathways. It is an important component of the ab5 toxin family where one A subunit and five B subunits form a complex to enter cells. The toxin binds to cell surface glycoproteins facilitating its uptake and subsequent inhibition of G-protein-coupled receptor (GPCR) signaling. This action interferes with a variety of intracellular processes ultimately leading to altered immune regulation and enhanced bacterial colonization.

Pathways

Pertussis toxin plays a significant role in the intracellular signaling pathways especially impacting the GPCR pathways. The toxin's catalytic subunit modifies the alpha subunit of inhibitory G-proteins (Gi) which prevents normal signal transduction from the GPCRs. This dysregulation impairs processes such as cyclic AMP (cAMP) regulation and immune response modulation. In the GPCR pathway B. pertussis toxin shows close functional relationships with other G-protein coupled components.

Pertussis toxin significantly contributes to the development of whooping cough a respiratory disease that can lead to severe coughing fits. This toxin affects various host immune proteins like chemokine receptors complicating immune response against the Bordetella pertussis bacterium. Additionally its action on the GPCR signaling pathway implicates it in other related respiratory conditions making pertussis toxin a pivotal target for therapeutic interventions and vaccine development against whooping cough.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane.
See full target information ptxB

Additional targets

Bordetella pertussis toxin subunit S1,Bordetella pertussis toxin subunit S3,Bordetella pertussis toxin subunit S4,Bordetella pertussis toxin subunit S5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Vaccine 14:359-68 PubMed8735545

1996

The effect of formaldehyde, hydrogen peroxide and genetic detoxification of pertussis toxin on epitope recognition by murine monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

P H Ibsen
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