AB188414

Anti-Bordetella pertussis toxin antibody

Name: Anti-Bordetella pertussis toxin antibody (ab188414) | Abcam
Brand: Abcam Limited
SKU: AB188414
Price: 390 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal TOX1 antibody. Suitable for IP, ELISA, Dot, WB, FuncS (Neut/Block) and reacts with Bordetella pertussis samples. Cited in 3 publications.

View Alternative Names

BP3783, ptxA, Pertussis toxin subunit 1, PTX S1, Islet-activating protein S1, NAD-dependent ADP-ribosyltransferase, IAP S1

3 Images

ELISA - Anti-Bordetella pertussis toxin antibody (AB188414)

ELISA

Supplier Data

ELISA - Anti-Bordetella pertussis toxin antibody (AB188414)

ELISA analysis of Bordetella pertussis toxin/ptxD, using ab188414 at 1/12500 dilution. Plate was coated with indicated amounts of pertussis toxin per well and 100 μl of ab188414 was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Color was developed with TMB as substrate. Dynamic range was 100 pg to 10 ng under these conditions.

ELISA

Supplier Data

ELISA - Anti-Bordetella pertussis toxin antibody (AB188414)

ELISA analysis of Bordetella pertussis toxin/ptxD, using ab188414. Plate was coated with 100 μg of pertussis toxin per well and 100 μl of ab188414 at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Color was developed with TMB as substrate.

Supplier Data

Western blot - Anti-Bordetella pertussis toxin antibody (AB188414)

The toxin consists of five subunits as indicated by S1 to S5.

All lanes:

Western blot - Anti-Bordetella pertussis toxin antibody (ab188414) at 1/2000 dilution

Lane 1:

Culture medium of Bordetella pertussis

Lane 2:

Purified Bordetella pertussis toxin at 0.01 µg

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Bordetella pertussis

Applications

Dot, WB, ELISA, FuncS (Neut/Block), IP

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bordetella pertussis": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

pH: 6 - 8.5 Preservative: 0.09% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bordetella pertussis toxin often called pertussis toxin or PTX functions mechanically as an exotoxin produced by Bordetella pertussis. This toxin has a molecular mass of around 105 kDa and comes from the bacterium responsible for whooping cough. It expresses itself in various host cells after the bacterium releases it into the respiratory tract during infection. The pertussis toxin consists of five subunits S1 through S5 which work together to impair host cellular functions by catalyzing the ADP-ribosylation of G-proteins.

Biological function summary

Pertussis toxin disrupts host immune responses and cellular signaling pathways. It is an important component of the ab5 toxin family where one A subunit and five B subunits form a complex to enter cells. The toxin binds to cell surface glycoproteins facilitating its uptake and subsequent inhibition of G-protein-coupled receptor (GPCR) signaling. This action interferes with a variety of intracellular processes ultimately leading to altered immune regulation and enhanced bacterial colonization.

Pathways

Pertussis toxin plays a significant role in the intracellular signaling pathways especially impacting the GPCR pathways. The toxin's catalytic subunit modifies the alpha subunit of inhibitory G-proteins (Gi) which prevents normal signal transduction from the GPCRs. This dysregulation impairs processes such as cyclic AMP (cAMP) regulation and immune response modulation. In the GPCR pathway B. pertussis toxin shows close functional relationships with other G-protein coupled components.

Pertussis toxin significantly contributes to the development of whooping cough a respiratory disease that can lead to severe coughing fits. This toxin affects various host immune proteins like chemokine receptors complicating immune response against the Bordetella pertussis bacterium. Additionally its action on the GPCR signaling pathway implicates it in other related respiratory conditions making pertussis toxin a pivotal target for therapeutic interventions and vaccine development against whooping cough.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors.

See full target information ptxA

Additional targets

ptxB,ptxC,ptxE,ptxD,ptxA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:3380 PubMed35697676

2022

NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda M Gleixner,Brandie Morris Verdone,Charlton G Otte,Eric N Anderson,Nandini Ramesh,Olivia R Shapiro,Jenna R Gale,Jocelyn C Mauna,Jacob R Mann,Katie E Copley,Elizabeth L Daley,Juan A Ortega,Maria Elena Cicardi,Evangelos Kiskinis,Julia Kofler,Udai B Pandey,Davide Trotti,Christopher J Donnelly

Molecular microbiology 117:1173-1195 PubMed35344242

2022

Aluminum hydroxide adjuvant diverts the uptake and trafficking of genetically detoxified pertussis toxin to lysosomes in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Jaldin-Fincati,Serene Moussaoui,Maria Cecilia Gimenez,Cheuk Y Ho,Charlene E Lancaster,Roberto Botelho,Fernando Ausar,Roger Brookes,Mauricio Terebiznik

Nature neuroscience 24:19-23 PubMed33318667

2020

Microglial G-dependent dynamics regulate brain network hyperexcitability.

Applications

Unspecified application

Species

Unspecified reactive species

Mario Merlini,Victoria A Rafalski,Keran Ma,Keun-Young Kim,Eric A Bushong,Pamela E Rios Coronado,Zhaoqi Yan,Andrew S Mendiola,Elif G Sozmen,Jae Kyu Ryu,Matthias G Haberl,Matthew Madany,Daniel Naranjo Sampson,Mark A Petersen,Sophia Bardehle,Reshmi Tognatta,Terry Dean,Rosa Meza Acevedo,Belinda Cabriga,Reuben Thomas,Shaun R Coughlin,Mark H Ellisman,Jorge J Palop,Katerina Akassoglou

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Bordetella pertussis toxin antibody

ELISA - Anti-Bordetella pertussis toxin antibody (AB188414)

ELISA - Anti-Bordetella pertussis toxin antibody (AB188414)

Western blot - Anti-Bordetella pertussis toxin antibody (AB188414)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

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