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AB37574

Anti-Bordetella pertussis toxin/ptxD antibody [63.1G9] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal TOX1 antibody. Carrier free. Suitable for ELISA, WB, Neut and reacts with Bordetella pertussis samples. Cited in 1 publication.

View Alternative Names

BP3783, ptxA, Pertussis toxin subunit 1, PTX S1, Islet-activating protein S1, NAD-dependent ADP-ribosyltransferase, IAP S1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

63.1G9

Isotype

IgG1

Carrier free

Yes

Reacts with

Bordetella pertussis

Applications

ELISA, WB, Neut

applications

Specificity

This product reacts to different epitope than ab37684, ab37686 and ab37687.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bordetella pertussis toxin often called pertussis toxin or PTX functions mechanically as an exotoxin produced by Bordetella pertussis. This toxin has a molecular mass of around 105 kDa and comes from the bacterium responsible for whooping cough. It expresses itself in various host cells after the bacterium releases it into the respiratory tract during infection. The pertussis toxin consists of five subunits S1 through S5 which work together to impair host cellular functions by catalyzing the ADP-ribosylation of G-proteins.
Biological function summary

Pertussis toxin disrupts host immune responses and cellular signaling pathways. It is an important component of the ab5 toxin family where one A subunit and five B subunits form a complex to enter cells. The toxin binds to cell surface glycoproteins facilitating its uptake and subsequent inhibition of G-protein-coupled receptor (GPCR) signaling. This action interferes with a variety of intracellular processes ultimately leading to altered immune regulation and enhanced bacterial colonization.

Pathways

Pertussis toxin plays a significant role in the intracellular signaling pathways especially impacting the GPCR pathways. The toxin's catalytic subunit modifies the alpha subunit of inhibitory G-proteins (Gi) which prevents normal signal transduction from the GPCRs. This dysregulation impairs processes such as cyclic AMP (cAMP) regulation and immune response modulation. In the GPCR pathway B. pertussis toxin shows close functional relationships with other G-protein coupled components.

Pertussis toxin significantly contributes to the development of whooping cough a respiratory disease that can lead to severe coughing fits. This toxin affects various host immune proteins like chemokine receptors complicating immune response against the Bordetella pertussis bacterium. Additionally its action on the GPCR signaling pathway implicates it in other related respiratory conditions making pertussis toxin a pivotal target for therapeutic interventions and vaccine development against whooping cough.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors.
See full target information ptxA

Additional targets

ptxD,ptxB,ptxC,ptxE,ptxA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Vaccine 14:359-68 PubMed8735545

1996

The effect of formaldehyde, hydrogen peroxide and genetic detoxification of pertussis toxin on epitope recognition by murine monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

P H Ibsen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB37686

Anti-Bordetella pertussis toxin/ptxD antibody [56.2]

primary-antibodies

bordetella-pertussis-toxin-ptxd-antibody-562-ab37686

0

(0 reviews)

Alternative Product
Primary Antibodies

AB188414

Anti-Bordetella pertussis toxin/ptxD antibody

primary-antibodies

bordetella-pertussis-toxin-ptxd-antibody-ab188414

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(0 reviews)

Alternative Product
Primary Antibodies

AB37687

Anti-Bordetella pertussis toxin/ptxD antibody [47.1]

primary-antibodies

bordetella-pertussis-toxin-ptxd-antibody-471-ab37687

0

(0 reviews)

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