Rabbit Polyclonal TOX1 antibody. Suitable for IP, ELISA, Dot, WB, Neut and reacts with Bordetella pertussis samples. Cited in 2 publications.
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Publications
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- Molecular microbiology 117:1173-11952022Aluminum hydroxide adjuvant diverts the uptake and trafficking of genetically detoxified pertussis toxin to lysosomes in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJavier Jaldin-Fincati,Serene Moussaoui,Maria Cecilia Gimenez,Cheuk Y Ho,Charlene E Lancaster,Roberto Botelho,Fernando Ausar,Roger Brookes,Mauricio TerebiznikPubMed 35344242
- Nature neuroscience 24:19-232020Microglial G-dependent dynamics regulate brain network hyperexcitability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMario Merlini,Victoria A Rafalski,Keran Ma,Keun-Young Kim,Eric A Bushong,Pamela E Rios Coronado,Zhaoqi Yan,Andrew S Mendiola,Elif G Sozmen,Jae Kyu Ryu,Matthias G Haberl,Matthew Madany,Daniel Naranjo Sampson,Mark A Petersen,Sophia Bardehle,Reshmi Tognatta,Terry Dean,Rosa Meza Acevedo,Belinda Cabriga,Reuben Thomas,Shaun R Coughlin,Mark H Ellisman,Jorge J Palop,Katerina AkassoglouPubMed 33318667
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.09% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| IP | ELISA | Dot | WB | Neut | |
|---|---|---|---|---|---|
| Bordetella pertussis | Expected | Tested | Expected | Tested | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Bordetella pertussis | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Bordetella pertussis | Dilution info - | Notes - |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Bordetella pertussis | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Bordetella pertussis | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Bordetella pertussis | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors.
Additional Targets
ptxB, ptxC, ptxE, ptxD, ptxA
Alternative names
BP3783, ptxA, Pertussis toxin subunit 1, PTX S1, Islet-activating protein S1, NAD-dependent ADP-ribosyltransferase, IAP S1
Recommended products
Rabbit Polyclonal TOX1 antibody. Suitable for IP, ELISA, Dot, WB, Neut and reacts with Bordetella pertussis samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.09% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bordetella pertussis toxin often called pertussis toxin or PTX functions mechanically as an exotoxin produced by Bordetella pertussis. This toxin has a molecular mass of around 105 kDa and comes from the bacterium responsible for whooping cough. It expresses itself in various host cells after the bacterium releases it into the respiratory tract during infection. The pertussis toxin consists of five subunits S1 through S5 which work together to impair host cellular functions by catalyzing the ADP-ribosylation of G-proteins.
- Biological function summary
Pertussis toxin disrupts host immune responses and cellular signaling pathways. It is an important component of the Anti-CENPF antibody ab5 toxin family where one A subunit and five B subunits form a complex to enter cells. The toxin binds to cell surface glycoproteins facilitating its uptake and subsequent inhibition of G-protein-coupled receptor (GPCR) signaling. This action interferes with a variety of intracellular processes ultimately leading to altered immune regulation and enhanced bacterial colonization.
- Pathways
Pertussis toxin plays a significant role in the intracellular signaling pathways especially impacting the GPCR pathways. The toxin's catalytic subunit modifies the alpha subunit of inhibitory G-proteins (Gi) which prevents normal signal transduction from the GPCRs. This dysregulation impairs processes such as cyclic AMP (cAMP) regulation and immune response modulation. In the GPCR pathway B. pertussis toxin shows close functional relationships with other G-protein coupled components.
- Associated diseases and disorders
Pertussis toxin significantly contributes to the development of whooping cough a respiratory disease that can lead to severe coughing fits. This toxin affects various host immune proteins like chemokine receptors complicating immune response against the Bordetella pertussis bacterium. Additionally its action on the GPCR signaling pathway implicates it in other related respiratory conditions making pertussis toxin a pivotal target for therapeutic interventions and vaccine development against whooping cough.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Bordetella pertussis toxin/ptxD antibody (ab188414)
The toxin consists of five subunits as indicated by S1 to S5.
All lanes: Western blot - Anti-Bordetella pertussis toxin/ptxD antibody (ab188414) at 1/2000 dilution
Lane 1: Culture medium of Bordetella pertussis
Lane 2: Purified Bordetella pertussis toxin/ptxD at 0.01 µg
ELISA - Anti-Bordetella pertussis toxin/ptxD antibody (ab188414)
ELISA analysis of Bordetella pertussis toxin/ptxD, using ab188414. Plate was coated with 100 μg of pertussis toxin per well and 100 μl of ab188414 at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Color was developed with TMB as substrate.
ELISA - Anti-Bordetella pertussis toxin/ptxD antibody (ab188414)
ELISA analysis of Bordetella pertussis toxin/ptxD, using ab188414 at 1/12500 dilution. Plate was coated with indicated amounts of pertussis toxin per well and 100 μl of ab188414 was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Color was developed with TMB as substrate. Dynamic range was 100 pg to 10 ng under these conditions.
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