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AB67126

Anti-Borealin/CDCA8 antibody

2

(4 Reviews)

|

(2 Publications)

Mouse Polyclonal Borealin/CDCA8 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CDCA8.

View Alternative Names

PESCRG3, CDCA8, Borealin, Cell division cycle-associated protein 8, Dasra-B, Pluripotent embryonic stem cell-related gene 3 protein, hDasra-B

1 Images
Western blot - Anti-Borealin/CDCA8 antibody (AB67126)
  • WB

Unknown

Western blot - Anti-Borealin/CDCA8 antibody (AB67126)

All lanes:

Western blot - Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilution

Lane 1:

Borealin/CDCA8 transfected 293T cell lysate

Lane 2:

Untransfected 293T cell lysate

Secondary

All lanes:

Goat anti mouse IgG (H&L)-HRP conjugate secondary antibody at 1/2500 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Native Full Length Protein corresponding to Human CDCA8.

Q53HL2

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Alternative Products

Primary Antibodies

AB74473

Anti-Borealin/CDCA8 antibody

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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Borealin also known as CDCA8 is a component of the chromosomal passenger complex (CPC) with a mass of approximately 22 kDa. This protein facilitates proper chromosome segregation and cytokinesis during cell division. It is mainly expressed in proliferating cells especially in tissues undergoing frequent cell cycles like testis and tumor tissues. Borealin localizes to the centromeres and the spindle midzone during mitosis highlighting its mechanical involvement in maintaining chromosome alignment and proper cell division.
Biological function summary

Borealin serves as an important element in ensuring accurate mitosis. It functions as an integral part of the chromosomal passenger complex which also includes Aurora B kinase Survivin and INCENP. This complex regulates chromosome-microtubule interactions and cytokinesis. Borealin interacts with other CPC components to ensure stability and proper localization of the complex facilitating important mitotic functions. This highlights Borealin's biological role in maintaining genomic stability.

Pathways

Borealin integrates into the cell cycle and mitotic pathways. These pathways include the regulation of Aurora B kinase and its associated proteins such as Survivin and INCENP playing a critical role in chromosome condensation spindle assembly and cytokinesis. In the mitotic pathway Borealin's interaction with the CPC allows for precise coordination of mitotic events providing a framework for successful cell division and its associated processes.

Abnormalities in Borealin function are linked to cancer and chromosomal instability syndromes. Disrupted Borealin expression or function can lead to abnormal mitosis and contribute to tumorigenesis. Research suggests that Borealin alterations may relate to other proteins involved in cancer including Aurora B kinase influencing cancer progression through enhanced genomic instability or proliferation rates. Understanding Borealin's contribution to these diseases may offer new avenues for therapeutic exploration in targeting cancer cell division.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
See full target information CDCA8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 12:13437-13449 PubMed33408516

2020

Identification of Core Genes Involved in the Metastasis of Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Peng,Yahui Wang,Likai Mao,Fang Fang,Han Guan

Nature communications 7:13853 PubMed28004745

2016

PKCɛ switches Aurora B specificity to exit the abscission checkpoint.

Applications

ICC/IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Tanya Pike,Nicola Brownlow,Svend Kjaer,Jeremy Carlton,Peter J Parker
View all publications
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Product promise

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