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AB74473

Anti-Borealin/CDCA8 antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Borealin/CDCA8 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 3 publications.

View Alternative Names

PESCRG3, CDCA8, Borealin, Cell division cycle-associated protein 8, Dasra-B, Pluripotent embryonic stem cell-related gene 3 protein, hDasra-B

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Borealin/CDCA8 antibody (AB74473)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Borealin/CDCA8 antibody (AB74473)

Immunofluorescence of CDCA8 in Rat Kidney cells using ab74473 at 20 ug/ml.

Western blot - Anti-Borealin/CDCA8 antibody (AB74473)
  • WB

Unknown

Western blot - Anti-Borealin/CDCA8 antibody (AB74473)

Lane 1:

Western blot - Anti-Borealin/CDCA8 antibody (ab74473) at 1 µg/mL

Lane 2:

Western blot - Anti-Borealin/CDCA8 antibody (ab74473) at 2 µg/mL

All lanes:

Rat kidney lysate at 15 µg

Predicted band size: 31 kDa

Observed band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Borealin also known as CDCA8 is a component of the chromosomal passenger complex (CPC) with a mass of approximately 22 kDa. This protein facilitates proper chromosome segregation and cytokinesis during cell division. It is mainly expressed in proliferating cells especially in tissues undergoing frequent cell cycles like testis and tumor tissues. Borealin localizes to the centromeres and the spindle midzone during mitosis highlighting its mechanical involvement in maintaining chromosome alignment and proper cell division.
Biological function summary

Borealin serves as an important element in ensuring accurate mitosis. It functions as an integral part of the chromosomal passenger complex which also includes Aurora B kinase Survivin and INCENP. This complex regulates chromosome-microtubule interactions and cytokinesis. Borealin interacts with other CPC components to ensure stability and proper localization of the complex facilitating important mitotic functions. This highlights Borealin's biological role in maintaining genomic stability.

Pathways

Borealin integrates into the cell cycle and mitotic pathways. These pathways include the regulation of Aurora B kinase and its associated proteins such as Survivin and INCENP playing a critical role in chromosome condensation spindle assembly and cytokinesis. In the mitotic pathway Borealin's interaction with the CPC allows for precise coordination of mitotic events providing a framework for successful cell division and its associated processes.

Abnormalities in Borealin function are linked to cancer and chromosomal instability syndromes. Disrupted Borealin expression or function can lead to abnormal mitosis and contribute to tumorigenesis. Research suggests that Borealin alterations may relate to other proteins involved in cancer including Aurora B kinase influencing cancer progression through enhanced genomic instability or proliferation rates. Understanding Borealin's contribution to these diseases may offer new avenues for therapeutic exploration in targeting cancer cell division.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
See full target information CDCA8
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2277 PubMed40097392

2025

A CPC-shelterin-BTR axis regulates mitotic telomere deprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Romero-Zamora,Samuel Rogers,Ronnie Ren Jie Low,Scott G Page,Blake J E Lane,Shunya Kosaka,Andrew B Robinson,Lucy French,Noa Lamm,Fuyuki Ishikawa,Makoto T Hayashi,Anthony J Cesare

Journal of clinical laboratory analysis 36:e24633 PubMed36085568

2022

Upregulation of KIF18B facilitates malignant phenotype of esophageal squamous cell carcinoma by activating CDCA8/mTORC1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangliu Xie,Bo Wang,Wenjie Luo,Chen Li,Xunchao Jia

Nucleic acids research 46:6544-6560 PubMed29901724

2018

Cyclin-dependent kinase 1 activity coordinates the chromatin associated state of Oct4 during cell cycle in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Ji Kim,Jihoon Shin,Sangho Lee,Tae Wan Kim,Hyonchol Jang,Min Young Suh,Jae-Hwan Kim,In-Young Hwang,Deog Su Hwang,Eun-Jung Cho,Hong-Duk Youn
View all publications
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