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AB315147

Anti-Bovine Coronavirus antibody [8D1]

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Mouse Monoclonal Bovine Coronavirus Nucleoprotein antibody. Suitable for ICC/IF and reacts with Mouse samples.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Bovine Coronavirus antibody [8D1] (AB315147)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Coronavirus antibody [8D1] (AB315147)

Immunofluorescence labeling Bovine Coronovirus (BCV) in positive control cells (MDBK cells infected with BCV virus, strain Mebus). Primary antibody : Mouse anti-BCV Monoclonal antibody clone (8D1) at 5μg/ml. Secondary antibody : Goat-anti-Mouse IgG (H+L), FITC conjugated at 1μg/ml (Green). DAPI : Nuclear counterstain (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Coronavirus antibody [8D1] (AB315147)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Coronavirus antibody [8D1] (AB315147)

Immunofluorescence labeling Bovine Coronovirus (BCV) in negative control cells (MDBK cells) without virus infection. Primary antibody : Mouse anti-BCV Monoclonal antibody clone (8D1) at 5μg/ml. Secondary antibody : Goat-anti-Mouse IgG (H+L), FITC conjugated at 1μg/ml (Green). DAPI : Nuclear counterstain (Blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8D1

Isotype

IgG1

Carrier free

No

Reacts with

Bovine coronavirus

Applications

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Bovine coronavirus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bovine Coronavirus Nucleoprotein (N) which is also known as the nucleocapsid protein plays an important role in the viral replication and transcription cycle. This protein weighing approximately 50 kDa serves multiple functions including packaging the viral RNA genome into a ribonucleoprotein complex. It is highly conserved and predominantly found within the infected cells where the Bovine Coronavirus is present.
Biological function summary

The Bovine Coronavirus Nucleoprotein interacts aggressively with viral RNA and other viral proteins to facilitate efficient virus assembly and egress. It forms part of a complex with the viral membrane (M) protein supporting virion structure and stability. By binding specifically to the viral RNA it protects the RNA from degradation therefore ensuring viral replication occurs effectively within the host cell.

Pathways

The Bovine Coronavirus Nucleoprotein plays a role in virus assembly and replication pathways contributing to the production of viral components necessary for successful infection propagation. It is closely linked with the M protein through these pathways helping maintain the structural integrity of the virion. Additionally it interacts with host cellular pathways affecting the host cell's protein synthesis machinery facilitating viral replication over host processes.

The Bovine Coronavirus Nucleoprotein is critically linked to bovine respiratory and enteric infections commonly associated with calf diarrhea and winter dysentery in adult cattle. It shows interactions with other viral proteins such as the spike (S) protein which plays a role in the virus's ability to infect host cells. Better understanding this nucleoprotein and its associated pathways can be pivotal for developing effective treatments and preventive measures against these veterinary diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Bovine Coronavirus Nucleoprotein
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Product promise

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