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AB325992

Anti-Bovine Herpesvirus 1- gE antibody [1B5]

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Mouse Monoclonal Bovine Herpesvirus 1- gE antibody. Suitable for WB, ICC/IF and reacts with Recombinant full length protein - bovine alphaherpesvirus 1, bovine alphaherpesvirus 1 samples.
4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)

Immunofluorescence labeling Bovine Herpesvirus type 1 (BoHV1) in positive control (about 30% MDBK cells with virus infection). Primary antibody ab325992 at 4 ug/mL; Secondary antibody Goat anti-mouse IgG (H+L) FITC conjugated at 1 : 1000 (1 ug/mL) dilution (green); DAPI : Nuclear counterstain (blue).

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)

Immunofluorescence labeling Bovine Herpesvirus type 1 (BoHV1) in negative control (about 30% MDBK cells without virus infection). Primary antibody ab325992 at 4 ug/mL; Secondary antibody Goat anti-mouse IgG (H+L) FITC conjugated at 1 : 1000 (1 ug/mL) dilution (green); DAPI : Nuclear counterstain (blue).

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)

Immunofluorescence labeling Bovine Herpesvirus type 1 (BoHV1) in positive control (about 30% MDBK cells with virus infection). Primary antibody ab325992 at 4 ug/mL; Secondary antibody Goat anti-mouse IgG (H+L) FITC conjugated at 1 : 1000 (1 ug/mL) dilution (green); DAPI : Nuclear counterstain (blue).

Western blot - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)
  • WB

Supplier Data

Western blot - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)

Western Blot Assay : Primary antibody ab325992 at 0.1 ug/mL for 1 hr at room temperature, followed by a Goat anti-mouse IgG secondary at 1 : 2000 dilution for 1 hr at room temperature. Lane 1 : MW. Lane 2 : BoHV1 gE 1ug. Lane 3 : BoHV1 gE 0.1ug. Lane 4 : BoHV1 gB 1ug.

All lanes:

Western blot - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (ab325992) at 0.1 µg/mL

Lane 2:

BoHV1 gE at 1 µg

Lane 3:

BoHV1 gE at 0.1 µg

Lane 4:

BoHV1 gB at 1 µg

Secondary

All lanes:

Goat anti-mouse IgG at 1/2000 dilution

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1B5

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

bovine alphaherpesvirus 1

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - bovine alphaherpesvirus 1": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "bovine alphaherpesvirus 1": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "4 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C|-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Bovine Herpesvirus 1- gE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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