AB980

Anti-Bovine Papilloma Virus E2 antibody [1E4]

Name: Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980) | Abcam
Brand: Abcam Limited
SKU: AB980
Price: 525 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(5 Publications)

Mouse Monoclonal VE2 antibody. Suitable for WB, IP, ICC/IF and reacts with Recombinant full length protein - Bovine papillomavirus, Bovine papillomavirus samples. Cited in 5 publications.

View Alternative Names

Regulatory protein E2, E2

1 Images

Supplier Data

Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (AB980)

All lanes:

Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

Lane 1:

Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg

Lane 2:

Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg

Lane 3:

Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg

Lane 4:

Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E4

Isotype

IgG1

Carrier free

Reacts with

Bovine papillomavirus

Applications

IP, WB, ICC/IF

applications

Epitope

Not Determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bovine papillomavirus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "To isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "1hr, RT. Dilute in a diluent containing 1% BSA in PBS." }, "Recombinant full length protein - Bovine papillomavirus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "1hr, RT. Dilute in a diluent containing 2% non-fat dry milk in TBST. (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bovine Papilloma Virus E2 (BPV E2) is an important regulatory protein involved in the lifecycle of the Bovine Papilloma Virus. It also goes by alternate names such as E2 protein or Papilloma E2 antigen. The E2 protein has a mass of approximately 40 kDa. It is expressed during the early phase of viral infection within the nucleus of infected cells. BPV E2 plays a mechanical role by binding to DNA at specific sequences facilitating viral replication and transcription.

Biological function summary

BPV E2 functions as a transcription factor that regulates the expression of viral genes. It is part of a larger complex with other viral proteins such as E1 with which it cooperates to initiate viral replication. E2’s control over viral gene expression allows it to modulate the virus's ability to replicate and persist in host cells influencing the viral lifecycle and maintenance.

Pathways

BPV E2 is integral in the regulation of pathways related to viral replication and transcription. It is closely involved in the papillomavirus replication pathway interacting with proteins such as E1 to initiate DNA replication. Another relevant pathway includes transcriptional regulation where E2 controls the expression of various viral genes contributing to papillomavirus pathogenesis.

BPV E2 is connected to bovine papillomatosis a condition characterized by wart formation in cattle. This disorder arises due to the uncontrolled proliferation of infected cells mediated by the viral replication facilitated by BPV E2. Its interaction with the E1 protein is essential for the viral replication that leads to disease progression. Understanding E2 and its role offers potential insights into therapeutic targets using bovine antibodies and possibly developing anti-papilloma strategies in bovine species.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.

See full target information E2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 138:457-476 PubMed31011859

2019

Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Beatriz Garcia-Diaz,Corinne Bachelin,Fanny Coulpier,Gaspard Gerschenfeld,Cyrille Deboux,Violetta Zujovic,Patrick Charnay,Piotr Topilko,Anne Baron-Van Evercooren

Molecular and cellular biology 37: PubMed28069738

2017

FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Takayoshi Yamauchi,Masaaki Nishiyama,Toshiro Moroishi,Atsuki Kawamura,Keiichi I Nakayama

Endocrinology 157:3682-95 PubMed27501182

2016

Type 2 Deiodinase Disruption in Astrocytes Results in Anxiety-Depressive-Like Behavior in Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara M L C Bocco,João Pedro Werneck-de-Castro,Kelen C Oliveira,Gustavo W Fernandes,Tatiana L Fonseca,Bruna P P Nascimento,Elizabeth A McAninch,Esther Ricci,Zsuzsanna Kvárta-Papp,Csaba Fekete,Maria Martha Bernardi,Balázs Gereben,Antonio C Bianco,Miriam O Ribeiro

Nature medicine 18:436-40 PubMed22366949

2012

Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL.

Applications

ChIP

Species

Unspecified reactive species

Giusy Della Gatta,Teresa Palomero,Arianne Perez-Garcia,Alberto Ambesi-Impiombato,Mukesh Bansal,Zachary W Carpenter,Kim De Keersmaecker,Xavier Sole,Luyao Xu,Elisabeth Paietta,Janis Racevskis,Peter H Wiernik,Jacob M Rowe,Jules P Meijerink,Andrea Califano,Adolfo A Ferrando

Journal of virology 73:4670-7 PubMed10233926

1999

Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

R Kurg,J Parik,E Juronen,T Sedman,A Abroi,I Liiv,U Langel,M Ustav

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bovine Papilloma Virus E2 antibody [1E4]

Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (AB980)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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