AB1072

Anti-Bovine Papilloma Virus E2 antibody [5H4]

Name: Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072) | Abcam
Brand: Abcam Limited
SKU: AB1072
Price: 515 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal VE2 antibody. Suitable for ICC/IF, IP, ELISA and reacts with Recombinant full length protein - Bovine papillomavirus, Bovine papillomavirus samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Deltapapillomavirus 4 E2.

View Alternative Names

Regulatory protein E2, E2

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (AB1072)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (AB1072)

Immunofluorescent staining of U2OS cells expressing BPV E2 protein with ab1072. The cells were counter-stained with DAPI, and the upper shows an image taken with illumination at both 460 nm and 488 nm, whereas the lower image shows illumination at 488 nm.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5H4

Isotype

IgG1

Carrier free

Reacts with

Bovine papillomavirus

Applications

ELISA, ICC/IF, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Deltapapillomavirus 4 E2.

P03122

Epitope

Conformational

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bovine papillomavirus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Bovine papillomavirus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bovine Papilloma Virus E2 (BPV E2) is an important regulatory protein involved in the lifecycle of the Bovine Papilloma Virus. It also goes by alternate names such as E2 protein or Papilloma E2 antigen. The E2 protein has a mass of approximately 40 kDa. It is expressed during the early phase of viral infection within the nucleus of infected cells. BPV E2 plays a mechanical role by binding to DNA at specific sequences facilitating viral replication and transcription.

Biological function summary

BPV E2 functions as a transcription factor that regulates the expression of viral genes. It is part of a larger complex with other viral proteins such as E1 with which it cooperates to initiate viral replication. E2’s control over viral gene expression allows it to modulate the virus's ability to replicate and persist in host cells influencing the viral lifecycle and maintenance.

Pathways

BPV E2 is integral in the regulation of pathways related to viral replication and transcription. It is closely involved in the papillomavirus replication pathway interacting with proteins such as E1 to initiate DNA replication. Another relevant pathway includes transcriptional regulation where E2 controls the expression of various viral genes contributing to papillomavirus pathogenesis.

BPV E2 is connected to bovine papillomatosis a condition characterized by wart formation in cattle. This disorder arises due to the uncontrolled proliferation of infected cells mediated by the viral replication facilitated by BPV E2. Its interaction with the E1 protein is essential for the viral replication that leads to disease progression. Understanding E2 and its role offers potential insights into therapeutic targets using bovine antibodies and possibly developing anti-papilloma strategies in bovine species.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.

See full target information E2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Veterinary dermatology 32:268-e74 PubMed33908122

2021

A case series of canine cutaneous inverted papilloma with one case showing evidence of recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa M S A Boehm,Sonya Bettenay,Wolf von Bomhard,Monir Majzoub-Altweck,Christoph J Klinger,Ralf S Mueller

Analytical and bioanalytical chemistry 410:3885-3903 PubMed29675707

2018

A rapid solution-based method for determining the affinity of heroin hapten-induced antibodies to heroin, its metabolites, and other opioids.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar B Torres,Alexander J Duval,Agnieszka Sulima,Joshua F G Antoline,Arthur E Jacobson,Kenner C Rice,Carl R Alving,Gary R Matyas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bovine Papilloma Virus E2 antibody [5H4]

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (AB1072)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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