AB79827

Anti-Bovine Serum Albumin antibody [1C12]

Name: Anti-Bovine Serum Albumin antibody [1C12] (ab79827) | Abcam
Brand: Abcam Limited
SKU: AB79827
Price: 330 USD
Availability: InStock

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(4 Publications)

Anti-Bovine Serum Albumin antibody [1C12] (ab79827) is a mouse monoclonal antibody detecting Bovine Serum Albumin in Western Blot, ELISA. Suitable for Cow.

- Trusted since 2009

View Alternative Names

Albumin, BSA, ALB

2 Images

Unknown

Western blot - Anti-Bovine Serum Albumin antibody [1C12] (AB79827)

Non-denaturing SDS-PAGE (7.5%).

All lanes:

Western blot - Anti-Bovine Serum Albumin antibody [1C12] (ab79827) at 1/2000 dilution

Lane 1:

Human Serum at 10 µL

Lane 2:

Bovine Serum at 10 µL

Lane 3:

Rabbit Serum at 10 µL

Secondary

All lanes:

Goat anti-mouse IgG (H+L) peroxidase conjugate at 1/4000 dilution

Predicted band size: 69 kDa

Observed band size: 67 kDa

false

Exposure time: 1min

ELISA - Anti-Bovine Serum Albumin antibody [1C12] (AB79827)

ELISA

Unknown

ELISA - Anti-Bovine Serum Albumin antibody [1C12] (AB79827)

Graph showing comparison of the reactivity of ab79287 with Bovine Serum Albumin, Ovalbumin, Rabbit Serum Albumin and Human Serum Albumin.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C12

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Cow

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts specifically with BSA. It does not cross react with Human Serum Albumin or Albumin from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/20000 - 1/100000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Cow": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/20000 - 1/100000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rabbit": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/20000 - 1/100000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Bovine Serum Albumin antibody [1C12] (ab79827) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), ELISA in Cow samples.

What is the molecular weight of Bovine Serum Albumin?
Anti-Bovine Serum Albumin [1C12] (ab79827) specifically detects a band for Bovine Serum Albumin (UniProt: P02769) at a molecular weight of 69kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.

Biological function summary

Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.

Pathways

Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.

Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed : 22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).

See full target information Albumin

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2414358 PubMed39746855

2025

ECSIT-X4 is Required for Preventing Pressure Overload-Induced Cardiac Hypertrophy via Regulating Mitochondrial STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Lu,Tingting Tong,Haoliang Sun,Yi Chen,Yongfeng Shao,Pengxi Shi,Linli Que,Li Liu,Guoqing Zhu,Qi Chen,Chuanfu Li,Jiantao Li,Shuo Yang,Yuehua Li

Cardiovascular diabetology 23:369 PubMed39420345

2024

Endothelial cells derived extracellular vesicles promote diabetic arterial calcification via circ_0008362/miR-1251-5p/Runx2 axial.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Lin,Sha-Qi He,Su-Kang Shan,Feng Xu,Feng Wu,Fu-Xing-Zi Li,Ming-Hui Zheng,Li-Min Lei,Jia-Yue Duan,Yun-Yun Wu,Yan-Lin Wu,Ke-Xin Tang,Rong-Rong Cui,Bei Huang,Jun-Jie Yang,Xiao-Bo Liao,Jun Liu,Ling-Qing Yuan

Chinese medical journal 137:1351-1359 PubMed38721807

2024

Direct reprogramming of fibroblasts into functional hepatocytes via CRISPRa activation of endogenous Gata4 and Foxa3.

Applications

Unspecified application

Species

Unspecified reactive species

Jiacheng Li,Ruopu Li,Xue Bai,Wenlong Zhang,Yu Nie,Shengshou Hu

Cell reports. Medicine 2:100446 PubMed34841293

2021

Vaccine targeting ANGPTL3 ameliorates dyslipidemia and associated diseases in mouse models of obese dyslipidemia and familial hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hirotaka Fukami,Jun Morinaga,Hironori Nakagami,Hiroki Hayashi,Yusuke Okadome,Eiji Matsunaga,Tsuyoshi Kadomatsu,Haruki Horiguchi,Michio Sato,Taichi Sugizaki,Takashige Kuwabara,Keishi Miyata,Masashi Mukoyama,Ryuichi Morishita,Yuichi Oike

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bovine Serum Albumin antibody [1C12]

Western blot - Anti-Bovine Serum Albumin antibody [1C12] (AB79827)

ELISA - Anti-Bovine Serum Albumin antibody [1C12] (AB79827)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise