AB9092

Anti-Bovine Serum Albumin antibody [5F9]

Name: Anti-Bovine Serum Albumin antibody [5F9] (ab9092) | Abcam
Brand: Abcam Limited
SKU: AB9092

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(4 Publications)

Mouse Monoclonal Albumin antibody. Suitable for WB, ELISA and reacts with Cow, Purified native protein - Cow samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow Albumin.

View Alternative Names

Albumin, BSA, ALB

1 Images

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Western blot - Anti-Bovine Serum Albumin antibody [5F9] (AB9092)

Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1 : 100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1 : 5000 in PBS and is reacted for 1.5 h at room temperature.

Detection occurs using a 1 : 5000 dilution of IRDye800^TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.

Detection occurs using a 1 : 5000 dilution of IRDye800

All lanes:

Western blot - Anti-Bovine Serum Albumin antibody [5F9] (ab9092)

Predicted band size: 69 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5F9

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Cow

Applications

WB, ELISA

applications

Immunogen

Native Full Length Protein corresponding to Cow Albumin. The exact immunogen used to generate this antibody is proprietary information.

P02769

Specificity

The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Chicken": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/120000", "ELISA-species-notes": "" }, "Purified native protein - Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Bovine Serum Albumin monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography and reacts with bovine serum albumin.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.

Biological function summary

Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.

Pathways

Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.

Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed : 22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).

See full target information Albumin

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Analytical and bioanalytical chemistry 415:4173-4184 PubMed36369591

2022

Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Duong,Simin Maleknia,David Clases,Andrew Minett,Matthew P Padula,Philip A Doble,Raquel Gonzalez de Vega

Scientific reports 6:27255 PubMed27251104

2016

Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions.

Applications

Species

Unspecified reactive species

Anton G Kutikhin,Elena A Velikanova,Rinat A Mukhamadiyarov,Tatiana V Glushkova,Vadim V Borisov,Vera G Matveeva,Larisa V Antonova,Dmitriy E Filip'ev,Alexey S Golovkin,Daria K Shishkova,Andrey Yu Burago,Alexey V Frolov,Viktor Yu Dolgov,Olga S Efimova,Anna N Popova,Valentina Yu Malysheva,Alexandr A Vladimirov,Sergey A Sozinov,Zinfer R Ismagilov,Dmitriy M Russakov,Alexander A Lomzov,Dmitriy V Pyshnyi,Anton K Gutakovsky,Yuriy A Zhivodkov,Evgeniy A Demidov,Sergey E Peltek,Viatcheslav F Dolganyuk,Olga O Babich,Evgeniy V Grigoriev,Elena B Brusina,Olga L Barbarash,Arseniy E Yuzhalin

The Journal of biological chemistry 289:30990-1000 PubMed25253696

2014

Molecular mechanisms of Alzheimer disease protection by the A673T allele of amyloid precursor protein.

Applications

Unspecified application

Species

Unspecified reactive species

Janice A Maloney,Travis Bainbridge,Amy Gustafson,Shuo Zhang,Roxanne Kyauk,Pascal Steiner,Marcel van der Brug,Yichin Liu,James A Ernst,Ryan J Watts,Jasvinder K Atwal

Colloids and surfaces. B, Biointerfaces 61:113-7 PubMed17703925

2007

Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Christopher Abell,Matthew A Cooper

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Bovine Serum Albumin antibody [5F9]

Western blot - Anti-Bovine Serum Albumin antibody [5F9] (AB9092)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise