Mouse Monoclonal Albumin antibody. Suitable for WB, ELISA and reacts with Cow, Purified native protein - Cow samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow Albumin.
Images
![Western blot - Anti-Bovine Serum Albumin antibody [5F9] (AB9092), expandable thumbnail](https://content.abcam.com/products/images/bovine-serum-albumin-antibody-5f9-ab9092--western-blot-img25214.gif "Western blot - Anti-Bovine Serum Albumin antibody [5F9] (AB9092)")
Publications
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- Analytical and bioanalytical chemistry 415:4173-41842022Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren Duong,Simin Maleknia,David Clases,Andrew Minett,Matthew P Padula,Philip A Doble,Raquel Gonzalez de VegaPubMed 36369591
- Scientific reports 6:272552016Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions.
- The Journal of biological chemistry 289:30990-10002014Molecular mechanisms of Alzheimer disease protection by the A673T allele of amyloid precursor protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanice A Maloney et. al.PubMed 25253696
- Colloids and surfaces. B, Biointerfaces 61:113-72007Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Li,Christopher Abell,Matthew A CooperPubMed 17703925
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Cow Albumin. The exact immunogen used to generate this antibody is proprietary information. Database link P02769
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Reactivity data
Select an application| WB | ELISA | |
|---|---|---|
| Human | Not recommended | Not recommended |
| Chicken | Not recommended | Not recommended |
| Cow | Tested | Expected |
| Purified native protein - Cow | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info 1/2000 - 1/10000 | Notes - |
Species Purified native protein - Cow | Dilution info 1/2000 - 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Human | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info 1/120000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Cow, Chicken, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed:22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Alternative names
Albumin, BSA, ALB
Recommended products
Mouse Monoclonal Albumin antibody. Suitable for WB, ELISA and reacts with Cow, Purified native protein - Cow samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow Albumin.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Cow Albumin. The exact immunogen used to generate this antibody is proprietary information. Database link P02769
- Clone number
- 5F9
- Purification technique
- Affinity purification Protein A
- Specificity
The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.
- Light chain type
- unknown
- Concentration
- Purification notes
Bovine Serum Albumin monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography and reacts with bovine serum albumin.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.
- Biological function summary
Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.
- Pathways
Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.
- Associated diseases and disorders
Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Bovine Serum Albumin antibody [5F9] (ab9092)
Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.
Detection occurs using a 1:5000 dilution of IRDye800TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.
Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.
Detection occurs using a 1:5000 dilution of IRDye800
All lanes: Western blot - Anti-Bovine Serum Albumin antibody [5F9] (ab9092)
Predicted band size: 69 kDa
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