AB186739

Anti-BPGM antibody [EPR15128]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal BPGM antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Bisphosphoglycerate mutase, BPGM, BPG-dependent PGAM, DPGM

4 Images

Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] (AB186739)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] (AB186739)

Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling BPGM with ab186739 at a 1/110 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

Supplier Data

Immunoprecipitation - Anti-BPGM antibody [EPR15128] (AB186739)

Western blot analysis on immunoprecipitation from 1) Human blood lysate and 2) PBS, labeling BPGM using 186739 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-BPGM antibody [EPR15128] (ab186739)

Predicted band size: 30 kDa

Observed band size: 30 kDa

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Supplier Data

Western blot - Anti-BPGM antibody [EPR15128] (AB186739)

All lanes:

Western blot - Anti-BPGM antibody [EPR15128] (ab186739) at 1/1000 dilution

All lanes:

Human placenta lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

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Supplier Data

Western blot - Anti-BPGM antibody [EPR15128] (AB186739)

All lanes:

Western blot - Anti-BPGM antibody [EPR15128] (ab186739) at 1/10000 dilution

All lanes:

Human blood lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

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Carrier free

Anti-BPGM antibody [EPR15128] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15128

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/110", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.11) activity.

See full target information BPGM

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40254393

2025

Reversible downregulation of HLA class I in adenoid cystic carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Annie Li,Bianca L Gonda,Elizabeth M Codd,Adam von Paternos,Dawn R Mitchell,Markus D Herrmann,Prinjali Kalyan,Samantha E Flynn,Thuc Q Dzu,Chengzhuo Gao,Edwin Zhang,Julia J Mendel,Julia C Thierauf,Peter M Sadow,Thomas Denize,Diane Yang,Florian J Fintelmann,Jo Anne Fordham,Ross D Merkin,Atul K Bhan,Yu-Chung Huang,Jeffrey Raizer,William C Faquin,Daniel L Faden,Xin Gao,Jong Chul Park,Lori J Wirth,Stefan T Kaluziak,A John Iafrate

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BPGM antibody [EPR15128]

Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] (AB186739)

Immunoprecipitation - Anti-BPGM antibody [EPR15128] (AB186739)

Western blot - Anti-BPGM antibody [EPR15128] (AB186739)

Western blot - Anti-BPGM antibody [EPR15128] (AB186739)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise