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AB250893

Anti-BPGM antibody [EPR15128] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal BPGM antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Bisphosphoglycerate mutase, BPGM, BPG-dependent PGAM, DPGM

4 Images
Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)

This data was developed using ab186739, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling BPGM with ab186739 at a 1/110 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

Immunoprecipitation - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)
  • IP

Supplier Data

Immunoprecipitation - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)

This data was developed using ab186739, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation from 1) Human blood lysate and 2) PBS, labeling BPGM using 186739 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-BPGM antibody [EPR15128] (<a href='/en-us/products/primary-antibodies/bpgm-antibody-epr15128-ab186739'>ab186739</a>)

Predicted band size: 30 kDa

Observed band size: 30 kDa

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Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)
  • WB

Supplier Data

Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)

This data was developed using ab186739, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BPGM antibody [EPR15128] (<a href='/en-us/products/primary-antibodies/bpgm-antibody-epr15128-ab186739'>ab186739</a>) at 1/1000 dilution

All lanes:

Human placenta lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

true

Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)
  • WB

Supplier Data

Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)

This data was developed using ab186739, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BPGM antibody [EPR15128] (<a href='/en-us/products/primary-antibodies/bpgm-antibody-epr15128-ab186739'>ab186739</a>) at 1/10000 dilution

All lanes:

Human blood lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15128

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250893 is the carrier-free version of ab186739.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.11) activity.
See full target information BPGM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com