Rabbit Recombinant Monoclonal BPGM antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunoprecipitation - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893), expandable thumbnail](https://content.abcam.com/products/images/bpgm-antibody-epr15128-bsa-and-azide-free-ab250893--immunoprecipitation-img68654.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)")
![Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893), expandable thumbnail](https://content.abcam.com/products/images/bpgm-antibody-epr15128-bsa-and-azide-free-ab250893--western-blot-img82065.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)")
![Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893), expandable thumbnail](https://content.abcam.com/products/images/bpgm-antibody-epr15128-bsa-and-azide-free-ab250893--western-blot-img81928.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)")
![Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893), expandable thumbnail](https://content.abcam.com/products/images/bpgm-antibody-epr15128-bsa-and-azide-free-ab250893--flow-cytometry-intracellular-img80238.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] - BSA and Azide free (AB250893)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.11) activity.
Alternative names
Bisphosphoglycerate mutase, BPGM, BPG-dependent PGAM, DPGM
Recommended products
Rabbit Recombinant Monoclonal BPGM antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15128
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250893 is the carrier-free version of Anti-BPGM antibody [EPR15128] ab186739.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893), expandable thumbnail](https://content.abcam.com/products/images/bpgm-antibody-epr15128-bsa-and-azide-free-ab250893--immunoprecipitation-img68654.jpg "Immunoprecipitation - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893)")
Immunoprecipitation - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893)
This data was developed using Anti-BPGM antibody [EPR15128] ab186739, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation from 1) Human blood lysate and 2) PBS, labeling BPGM using 186739 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
All lanes: Immunoprecipitation - Anti-BPGM antibody [EPR15128] (Anti-BPGM antibody [EPR15128] ab186739)
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa
![Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893), expandable thumbnail](https://content.abcam.com/products/images/bpgm-antibody-epr15128-bsa-and-azide-free-ab250893--western-blot-img82065.jpg "Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893)")
Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893)
This data was developed using Anti-BPGM antibody [EPR15128] ab186739, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-BPGM antibody [EPR15128] (Anti-BPGM antibody [EPR15128] ab186739) at 1/10000 dilution
All lanes: Human blood lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa
![Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893), expandable thumbnail](https://content.abcam.com/products/images/bpgm-antibody-epr15128-bsa-and-azide-free-ab250893--western-blot-img81928.jpg "Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893)")
Western blot - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893)
This data was developed using Anti-BPGM antibody [EPR15128] ab186739, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-BPGM antibody [EPR15128] (Anti-BPGM antibody [EPR15128] ab186739) at 1/1000 dilution
All lanes: Human placenta lysate at 20 µg
SecondaryAll lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa
![Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893), expandable thumbnail](https://content.abcam.com/products/images/bpgm-antibody-epr15128-bsa-and-azide-free-ab250893--flow-cytometry-intracellular-img80238.jpg "Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893)")
Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] - BSA and Azide free (ab250893)
This data was developed using Anti-BPGM antibody [EPR15128] ab186739, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling BPGM with Anti-BPGM antibody [EPR15128] ab186739 at a 1/110 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
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