Anti-BPTF / FALZ antibody [EPR25422-35] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BPTF / FALZ antibody [EPR25422-35] - BSA and Azide free (AB288166)

This data was developed using ab288159, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling BPTF / FALZ with ab288159 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] - BSA and Azide free (AB288166)

This data was developed using ab288159, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling BPTF / FALZ with ab288159 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human lung carcinoma (PMID : 26418899).The section was incubated with ab288159 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] - BSA and Azide free (AB288166)

This data was developed using ab288159, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling BPTF / FALZ with ab288159 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human astrocytoma (PMID : 30542695). The section was incubated with ab288159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] - BSA and Azide free (AB288166)

This data was developed using ab288159, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling BPTF / FALZ with ab288159 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human endometrial carcinoma. The section was incubated with ab288159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BPTF / FALZ antibody [EPR25422-35] - BSA and Azide free (AB288166)

This data was developed using ab288159, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling BPTF / FALZ with ab288159 at 1/50 (10.76 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• WB

Lab

Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] - BSA and Azide free (AB288166)

This data was developed using ab288159, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

The observed MW/expression profile is consistent with what has been described in the literature (PMID : 31498079).

Exposure time : Lane1 : 10 seconds; Lane2 : 5.5 seconds;

All lanes:

Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] (<a href='/en-us/products/primary-antibodies/bptf-falz-antibody-epr25422-35-ab288159'>ab288159</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 338 kDa

Observed band size: 320 kDa

false

• WB

Lab

Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] - BSA and Azide free (AB288166)

This data was developed using ab288159, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW/expression profile is consistent with what has been described in the literature (PMID : 31498079).

Exposure time : 37 seconds

All lanes:

Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] (<a href='/en-us/products/primary-antibodies/bptf-falz-antibody-epr25422-35-ab288159'>ab288159</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa transfected with BPTF siRNA 1 whole cell lysate at 20 µg

Lane 3:

HeLa transfected with BPTF siRNA 2 whole cell lysate at 20 µg

Lane 4:

HeLa transfected with BPTF siRNA 3 whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 338 kDa

Observed band size: 320 kDa

false