AB140661

Anti-Brachyury / Bry antibody [1H9A2]

Name: Anti-Brachyury / Bry antibody [1H9A2] Mouse monoclonal (ab140661) | Abcam
Brand: Abcam Limited
SKU: AB140661
Price: 430 USD
Availability: InStock

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(5 Publications)

Mouse Monoclonal Brachyury / Bry antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P and reacts with Human, Recombinant fragment samples. Cited in 5 publications.

View Alternative Names

T, TBXT, T-box transcription factor T, Brachyury protein, Protein T

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Brachyury / Bry with ab140661 at 1/200 dilution followed by DAB staining.

Flow Cytometry - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

Flow Cyt

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Flow Cytometry - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

Flow cytometric analysis of HeLa cells labeling Brachyury / Bry with ab140661 at 1/200 dilution (green); negative control (red).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Brachyury / Bry with ab140661 at 1/200 dilution followed by DAB staining.

ELISA - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

ELISA

Unknown

ELISA - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

ELISA analysis using ab140661. Antigen = Purified recombinant fragment, corresponding to amino acids 257-309 of Human Brachyury / Bry, expressed in E. Coli.

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Western blot - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

All lanes:

Western blot - Anti-Brachyury / Bry antibody [1H9A2] (ab140661) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

HEK293 cell lysate, transfected with recombinant Human Brachyury / Bry fragment (aa 257-309)

Predicted band size: 47 kDa

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Western blot - Anti-Brachyury / Bry antibody [1H9A2] (AB140661)

Expected MW is 31.2 kDa.

All lanes:

Western blot - Anti-Brachyury / Bry antibody [1H9A2] (ab140661) at 1/500 dilution

All lanes:

Recombinant Human Brachyury / Bry protein

Predicted band size: 47 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1H9A2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ELISA, WB, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Recombinant fragment": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR136145-8 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Brachyury protein also known as Bry or T acts as a transcription factor and belongs to the T-box family of genes. It consists of about 435 amino acids giving it an estimated mass of around 47 kDa. Brachyury plays a critical role in the development of the posterior mesoderm and nucleus pulposus during embryogenesis. It is strongly expressed in the notochord mesoderm and some tumor tissues. The protein's presence can be detected using specific assays such as Brachyury IHC (immunohistochemistry) and Brachyury stain which help identify Brachyury marker expression in both normal and pathological states.

Biological function summary

The Brachyury protein influences the morphogenetic movement in mesoderm development. It directly interacts with specific DNA sequences to regulate the transcription of target genes. Brachyury often operates as part of a larger transcriptional complex that manages the differentiation and migration of cells during embryogenesis. The gene's expression indicates mesodermal differentiation and is important for cell fate decisions within developing embryos.

Pathways

Brachyury plays a significant role in the Wnt signaling and the TGF-beta pathways. These pathways are essential in controlling cell proliferation and differentiation. Brachyury directly impacts the regulation of these pathways influencing other proteins like beta-catenin in Wnt signaling. This interconnectedness suggests its broader role in cellular communication and development processes potentially affecting gene expression across these pathways.

Brachyury has been associated with various cancers such as chordoma and other related tumors. Chordoma specifically involves the notochord where Brachyury marks the disease's presence. Other proteins like E-cadherin interact with Brachyury in tumor progression and metastasis. Understanding Brachyury's function helps in diagnosing and potentially targeting treatment options for these ailments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.

See full target information TBXT

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:9826-9836 PubMed34541823

2021

LINC00261 elevation inhibits angiogenesis and cell cycle progression of pancreatic cancer cells by upregulating SCP2 via targeting FOXP3.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zou,Xuanzeng Pei,Dan Xing,Xiaojun Wu,Shuai Chen

International journal of molecular sciences 20: PubMed31575031

2019

iPSC-Derived Embryoid Bodies as Models of c--Mutated Hereditary Papillary Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wook Hwang,Christophe Desterke,Olivier Féraud,Stephane Richard,Sophie Ferlicot,Virginie Verkarre,Jean Jacques Patard,Julien Loisel-Duwattez,Adlen Foudi,Frank Griscelli,Annelise Bennaceur-Griscelli,Ali G Turhan

Scientific reports 8:12960 PubMed30154523

2018

Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Liu,Kevin Van Bortle,Yue Zhang,Ming-Tao Zhao,Joe Z Zhang,Benjamin S Geller,Joshua J Gruber,Chao Jiang,Joseph C Wu,Michael P Snyder

Journal of neurosurgery 128:1428-1437 PubMed28753115

2017

Brachyury gene copy number gain and activation of the PI3K/Akt pathway: association with upregulation of oncogenic Brachyury expression in skull base chordoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ryohei Otani,Akitake Mukasa,Masahiro Shin,Mayu Omata,Shunsaku Takayanagi,Shota Tanaka,Keisuke Ueki,Nobuhito Saito

Oncotarget 7:11478-86 PubMed26862851

2016

Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Jana Jezkova,Jason S Williams,Filipe Pinto,Stephen J Sammut,Geraint T Williams,Simon Gollins,Ramsay J McFarlane,Rui Manuel Reis,Jane A Wakeman

View all publications

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