Rabbit Recombinant Monoclonal Brachyury / Bry antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-Brachyury / Bry antibody [54] (AB259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--western-blot-img175028.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Brachyury / Bry antibody [54] (AB259260)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170406.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178717.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260)")
![Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (AB259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--immunoprecipitation-img164817.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (AB259260)")
![Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [54] (AB259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--immunocytochemistry-immunofluorescence-img178669.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [54] (AB259260)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.
Alternative names
T, TBXT, T-box transcription factor T, Brachyury protein, Protein T
Recommended products
Rabbit Recombinant Monoclonal Brachyury / Bry antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 54
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Brachyury protein also known as Bry or T acts as a transcription factor and belongs to the T-box family of genes. It consists of about 435 amino acids giving it an estimated mass of around 47 kDa. Brachyury plays a critical role in the development of the posterior mesoderm and nucleus pulposus during embryogenesis. It is strongly expressed in the notochord mesoderm and some tumor tissues. The protein's presence can be detected using specific assays such as Brachyury IHC (immunohistochemistry) and Brachyury stain which help identify Brachyury marker expression in both normal and pathological states.
- Biological function summary
The Brachyury protein influences the morphogenetic movement in mesoderm development. It directly interacts with specific DNA sequences to regulate the transcription of target genes. Brachyury often operates as part of a larger transcriptional complex that manages the differentiation and migration of cells during embryogenesis. The gene's expression indicates mesodermal differentiation and is important for cell fate decisions within developing embryos.
- Pathways
Brachyury plays a significant role in the Wnt signaling and the TGF-beta pathways. These pathways are essential in controlling cell proliferation and differentiation. Brachyury directly impacts the regulation of these pathways influencing other proteins like beta-catenin in Wnt signaling. This interconnectedness suggests its broader role in cellular communication and development processes potentially affecting gene expression across these pathways.
- Associated diseases and disorders
Brachyury has been associated with various cancers such as chordoma and other related tumors. Chordoma specifically involves the notochord where Brachyury marks the disease's presence. Other proteins like E-cadherin interact with Brachyury in tumor progression and metastasis. Understanding Brachyury's function helps in diagnosing and potentially targeting treatment options for these ailments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Brachyury / Bry antibody [54] (ab259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--western-blot-img175028.jpg "Western blot - Anti-Brachyury / Bry antibody [54] (ab259260)")
Western blot - Anti-Brachyury / Bry antibody [54] (ab259260)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
All lanes: Western blot - Anti-Brachyury / Bry antibody [54] (ab259260) at 1/5000 dilution
All lanes: MUG-Chor1 (human sacral bone chordoma), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (ab259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170406.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (ab259260)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (ab259260)
Immunohistochemical analysis of paraffin-embedded Human chordoma tissue labeling Brachyury / Bry with ab259260 at 1/5000 (0.203 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Mainly nuclear staining on human chordoma. The section was incubated with ab259260 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with .
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (ab259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178717.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (ab259260)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (ab259260)
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Brachyury / Bry with ab259260 at 1/5000 (0.203 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: no staining on human lung (PMID: 23456319). The section was incubated with ab259260 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with .
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (ab259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--immunoprecipitation-img164817.jpg "Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (ab259260)")
Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (ab259260)
Brachyury / Bry was immunoprecipitated from 0.35 mg MUG-Chor1 (human sacral bone chordoma), whole cell lysate 10ug with ab259260 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259260 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/10000 dilution.
Lane 1: MUG-Chor1 (human sacral bone chordoma), whole cell lysate 10ug
Lane 2: ab259260 IP in MUG-Chor1 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Insulin antibody [RM1019] ab282459 in MUG-Chor1 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
All lanes: Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (ab259260)
Predicted band size: 47 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [54] (ab259260), expandable thumbnail](https://content.abcam.com/products/images/brachyury-bry-antibody-54-ab259260--immunocytochemistry-immunofluorescence-img178669.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [54] (ab259260)")
Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [54] (ab259260)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized MUG-Chor1 cells labelling Brachyury / Bry with ab259260 at 1/50 (20.28 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MUG-Chor1 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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