AB20680

Anti-Brachyury / Bry antibody

(5 Reviews)

(61 Publications)

Rabbit Polyclonal Brachyury / Bry antibody. Suitable for WB and reacts with Human samples. Cited in 61 publications.

View Alternative Names

T, TBXT, T-box transcription factor T, Brachyury protein, Protein T

2 Images

Lab

Western blot - Anti-Brachyury / Bry antibody (AB20680)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab20680 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Brachyury / Bry antibody (ab20680) at 1 µg/mL

Lane 1:

MUG-Chor1 (human sacral bone chordoma) at 10 µg

Lane 2:

MCF7 whole cell lysate (negative control) at 15 µg

Lane 3:

Human embryonic stem cells (pluripotent) (negative control) at 10 µg

Lane 4:

Human embryonic stem cells (mesoderm) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

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Exposure time: 4min

Project4578****

Western blot - Anti-Brachyury / Bry antibody (AB20680)

ab20680 recognizes a band of 53 kDa in Murine Embryonic Stem Cells differentiated to express mesoderm markers. This corresponds in size to the Brachyury protein which has a predicted molecular weight of 47 kDa. A band of 75 kDa has also been observed, although we are unsure as to the identity of this band.

All lanes:

Western blot - Anti-Brachyury / Bry antibody (ab20680) at 1 µg/mL

All lanes:

Whole Cell Lysate from Murine ES Cells Differentiated to Express Mesoderm Markers at 15 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 47 kDa

Observed band size: 53 kDa,75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used to raise this antibody has 81% homology with the Mouse Brachyury protein. Some customers have successfully used ab20680 on mouse samples, however we have not been successful detecting Brachyury in this species in our own testing and therefore cannot guarantee mouse reactivity. Please contact Abcam Scientific Support for more information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Brachyury protein also known as Bry or T acts as a transcription factor and belongs to the T-box family of genes. It consists of about 435 amino acids giving it an estimated mass of around 47 kDa. Brachyury plays a critical role in the development of the posterior mesoderm and nucleus pulposus during embryogenesis. It is strongly expressed in the notochord mesoderm and some tumor tissues. The protein's presence can be detected using specific assays such as Brachyury IHC (immunohistochemistry) and Brachyury stain which help identify Brachyury marker expression in both normal and pathological states.

Biological function summary

The Brachyury protein influences the morphogenetic movement in mesoderm development. It directly interacts with specific DNA sequences to regulate the transcription of target genes. Brachyury often operates as part of a larger transcriptional complex that manages the differentiation and migration of cells during embryogenesis. The gene's expression indicates mesodermal differentiation and is important for cell fate decisions within developing embryos.

Pathways

Brachyury plays a significant role in the Wnt signaling and the TGF-beta pathways. These pathways are essential in controlling cell proliferation and differentiation. Brachyury directly impacts the regulation of these pathways influencing other proteins like beta-catenin in Wnt signaling. This interconnectedness suggests its broader role in cellular communication and development processes potentially affecting gene expression across these pathways.

Brachyury has been associated with various cancers such as chordoma and other related tumors. Chordoma specifically involves the notochord where Brachyury marks the disease's presence. Other proteins like E-cadherin interact with Brachyury in tumor progression and metastasis. Understanding Brachyury's function helps in diagnosing and potentially targeting treatment options for these ailments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.

See full target information TBXT

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Publications (61)

Recent publications for all applications. Explore the full list and refine your search

Histochemistry and cell biology 163:76 PubMed40658268

2025

Differentiation to insulin-positive cells from human amnion epithelial cells using a pancreatic development mimicry protocol.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Martínez-Rodríguez,Jonathan Salazar-Alonso,Axel Castro-Abrego,Daniela Ávila-González,Omar Martínez-Alarcón,Anayansi Molina-Hernández,Alejandro Martínez-Juárez,Héctor Salvador Godoy-Morales,Diana Elizabeth Lara-Barragán,Wendy Portillo,Nestor Emmanuel Díaz-Martínez,Guadalupe García-López,Nestor Fabián Díaz

Frontiers in cell and developmental biology 12:1395723 PubMed38887514

2024

Optimizing Nodal, Wnt and BMP signaling pathways for robust and efficient differentiation of human induced pluripotent stem cells to intermediate mesoderm cells.

Applications

Unspecified application

Species

Unspecified reactive species

Esmeralda Magro-Lopez,Elena Vazquez-Alejo,María de la Sierra Espinar-Buitrago,María Ángeles Muñoz-Fernández

Biochemical genetics 63:698-712 PubMed38509423

2024

Design of an In Vitro Model for Epithelial-to-Mesenchymal Transition in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanhui Zhang,Ling Bi,Quanyao Li,Liqiu Yao,Xiao Wang,Hui Liu,Jun Shi

Stem cell research 69:103072 PubMed37001364

2023

Generation of a set of induced pluripotent stem cell lines from two Alzheimer disease patients carrying APOE4 (MLUi007-J; MLUi008-A) and healthy old donors carrying APOE3 (MLUi009-A; MLUi010-B) to study APOE in aging and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Jung,Carla Hartmann,Toni Ehrhardt,Lisa-Maria Peter,Chaudhry Luqman Abid,Bernadette Harwardt,Jana Hirschfeld,Claudia Claus,Undine Haferkamp,Ole Pless,Marina Nastainczyk-Wulf,Astrid Kehlen,Dietmar Schlote,Insa S Schroder,Dan Rujescu

Stem cell research 61:102758 PubMed35364395

2022

Characterization of an induced pluripotent stem cell line (UMi040-A) bearing an auditory neuropathy spectrum disorder-associated variant in TMEM43.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Ciao Tang,Marie V Roche,Se Young Um,Nicholas C Gosstola,Min Young Kim,Byung Yoon Choi,Derek M Dykxhoorn,Xue Zhong Liu

Cell proliferation 55:e13201 PubMed35141969

2022

In vivo development and single-cell transcriptome profiling of human brain organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Shichao Huang,Fei Huang,Huiying Zhang,Yongfeng Yang,Juan Lu,Jiadong Chen,Li Shen,Gang Pei

The journal of cardiovascular aging 1: PubMed34790975

2021

S-nitrosoglutathione reductase (GSNOR) deficiency accelerates cardiomyocyte differentiation of induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro G Salerno,Amarylis C B A Wanschel,Raul A Dulce,Konstantinos E Hatzistergos,Wayne Balkan,Joshua M Hare

Biology open 10: PubMed33589441

2021

Single-cell sequencing reveals the potential oncogenic expression atlas of human iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Minglin Ou,Min Zhao,Chunhong Li,Donge Tang,Yong Xu,Weier Dai,Weiguo Sui,Yue Zhang,Zhen Xiang,Chune Mo,Hua Lin,Yong Dai

NPJ Regenerative medicine 5:18 PubMed33298947

2020

IFI16 promotes human embryonic stem cell trilineage specification through interaction with p53.

Applications

Unspecified application

Species

Unspecified reactive species

Qian He,Zubiao Wu,Wei Yang,Doukou Jiang,Chaofeng Hu,Xiaofei Yang,Ning Li,Furong Li

Stem cells and development 29:1179-1189 PubMed32723003

2020

The H-Y Antigen in Embryonic Stem Cells Causes Rejection in Syngeneic Female Recipients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Hu,Simon T Kueppers,Nigel G Kooreman,Alessia Gravina,Dong Wang,Grigol Tediashvili,Stephan Schlickeiser,Marco Frentsch,Christos Nikolaou,Andreas Thiel,Sivan Marcus,Sigrid Fuchs,Joachim Velden,Hermann Reichenspurner,Hans-Dieter Volk,Tobias Deuse,Sonja Schrepfer

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