AB137367

Anti-BRAF antibody

Name: Anti-BRAF antibody (ab137367) | Abcam
Brand: Abcam Limited
SKU: AB137367
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal BRAF antibody. Suitable for WB, IP and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BRAF aa 200-500.

View Alternative Names

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

2 Images

Supplier Data

Immunoprecipitation - Anti-BRAF antibody (AB137367)

Samples were separated by 10% SDS-PAGE. The immunoprecipitated B-Raf protein was detected by ab137367 diluted at 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-BRAF antibody (ab137367) at 3 µg

Lane 1:

HepG2 whole cell lysates at 30 µg

Lane 2:

HepG2 whole cell lysates with control IgG

Lane 3:

HepG2 whole cell lysates (+)

Secondary

All lanes:

Anti-Rabbit IgG (HRP)

Predicted band size: 84 kDa

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Supplier Data

Western blot - Anti-BRAF antibody (AB137367)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-BRAF antibody (ab137367) at 1/5000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

hB-Raf transfected 293T whole cell lysates at 30 µg

Predicted band size: 84 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human BRAF aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

P15056

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).

See full target information BRAF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal 37:3848-3863 PubMed28283560

2017

HDAC7 Ubiquitination by the E3 Ligase CBX4 Is Involved in Contextual Fear Conditioning Memory Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Jing,Wen-Hai Sui,Shuai Wang,Xu-Feng Xu,Rong-Rong Yuan,Xiao-Rong Chen,Hui-Xian Ma,Ying-Xiao Zhu,Jin-Kai Sun,Fan Yi,Zhe-Yu Chen,Yue Wang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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