Anti-BRAF antibody

• WB

Ap

Western blot - Anti-BRAF antibody (AB254565)

Primary incubation for 1 hour at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-BRAF antibody (ab254565) at 0.5 µg/mL

Lane 1:

Caco-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg

Lane 2:

Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg

Lane 4:

THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg

Secondary

All lanes:

Goat anti-Chicken HRP at 1/10000 dilution

Predicted band size: 84 kDa

false

• WB

Supplier Data

Western blot - Anti-BRAF antibody (AB254565)

Primary incubation for 1 hour at RT in 5% NFDM/TBST.

Lane 1:

Western blot - Anti-BRAF antibody (ab254565) at 1 µg/mL

Lane 2:

Western blot - Anti-BRAF antibody (ab254565) at 2 µg/mL

All lanes:

Human brain tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-Chicken HRP at 1/10000 dilution

Predicted band size: 84 kDa

false

• WB

Supplier Data

Western blot - Anti-BRAF antibody (AB254565)

Primary incubation for 1 hour at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-BRAF antibody (ab254565) at 0.5 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg

Lane 3:

A549 (human lung carcinoma cell line) cell lysate at 15 µg

Lane 4:

Caco-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg

Lane 5:

Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg

Lane 6:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

Lane 7:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg

Lane 8:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 15 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg

Lane 10:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

Lane 11:

SK-N-SH (human neuroblastoma cell line) cell lysate at 15 µg

Lane 12:

THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg

Lane 13:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg

Lane 14:

L1210 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-Chicken HRP at 1/10000 dilution

Predicted band size: 84 kDa

false