Chicken Polyclonal BRAF antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human BRAF aa 300-400.
Images
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BRAF aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link P15056
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Chicken | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Alternative names
BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
Recommended products
Chicken Polyclonal BRAF antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human BRAF aa 300-400.
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BRAF aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link P15056
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-BRAF antibody (ab254565)
Primary incubation for 1 hour at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-BRAF antibody (ab254565) at 0.5 µg/mL
Lane 1: Caco-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg
Lane 2: Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg
Lane 4: THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg
SecondaryAll lanes: Goat anti-Chicken HRP at 1/10000 dilution
Predicted band size: 84 kDa
Western blot - Anti-BRAF antibody (ab254565)
Primary incubation for 1 hour at RT in 5% NFDM/TBST.
Lane 1: Western blot - Anti-BRAF antibody (ab254565) at 1 µg/mL
Lane 2: Western blot - Anti-BRAF antibody (ab254565) at 2 µg/mL
All lanes: Human brain tissue lysate at 15 µg
SecondaryAll lanes: Goat anti-Chicken HRP at 1/10000 dilution
Predicted band size: 84 kDa
Western blot - Anti-BRAF antibody (ab254565)
Primary incubation for 1 hour at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-BRAF antibody (ab254565) at 0.5 µg/mL
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg
Lane 2: A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg
Lane 3: A549 (human lung carcinoma cell line) cell lysate at 15 µg
Lane 4: Caco-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg
Lane 5: Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Lane 7: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg
Lane 8: K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 15 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg
Lane 10: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg
Lane 11: SK-N-SH (human neuroblastoma cell line) cell lysate at 15 µg
Lane 12: THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg
Lane 13: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg
Lane 14: L1210 cell lysate at 15 µg
SecondaryAll lanes: Goat anti-Chicken HRP at 1/10000 dilution
Predicted band size: 84 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com