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AB85972

Anti-BRAF antibody

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(4 Publications)

Rabbit Polyclonal BRAF antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human BRAF aa 1-100.

View Alternative Names

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody (AB85972)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody (AB85972)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling B Raf with ab85972 at 1/200 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-BRAF antibody (AB85972)
  • IP

Unknown

Immunoprecipitation - Anti-BRAF antibody (AB85972)

Detection of B Raf by Western Blot of Immunprecipitate.
ab85972 at 1µg/ml staining B Raf in HeLa whole cell lysate immunoprecipitated using ab85972 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-BRAF antibody (ab85972)

Predicted band size: 84 kDa

false

Western blot - Anti-BRAF antibody (AB85972)
  • WB

Unknown

Western blot - Anti-BRAF antibody (AB85972)

All lanes:

Western blot - Anti-BRAF antibody (ab85972) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 84 kDa

Observed band size: 238 kDa,60 kDa,95 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human BRAF aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P15056

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab85972 was affinity purified using an epitope specific to B Raf immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).
See full target information BRAF
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112630 PubMed37300833

2023

Extrafollicular IgDCD27CXCR5CD11c DN3 B cells infiltrate inflamed tissues in autoimmune fibrosis and in severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Hugues Allard-Chamard,Naoki Kaneko,Alice Bertocchi,Na Sun,Julie Boucau,Hsiao-Hsuan Kuo,Jocelyn R Farmer,Cory Perugino,Vinay S Mahajan,Samuel J H Murphy,Katherine Premo,Thomas Diefenbach,Musie Ghebremichael,Grace Yuen,Alekhya Kotta,Zafer Akman,Mathias Lichterfeld,Bruce D Walker,Xu G Yu,Masafumi Moriyama,Takashi Maehara,Seiji Nakamura,John H Stone,Robert F Padera,Shiv Pillai

Cell 183:143-157.e13 PubMed32877699

2020

Loss of Bcl-6-Expressing T Follicular Helper Cells and Germinal Centers in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Kaneko,Hsiao-Hsuan Kuo,Julie Boucau,Jocelyn R Farmer,Hugues Allard-Chamard,Vinay S Mahajan,Alicja Piechocka-Trocha,Kristina Lefteri,Matthew Osborn,Julia Bals,Yannic C Bartsch,Nathalie Bonheur,Timothy M Caradonna,Josh Chevalier,Fatema Chowdhury,Thomas J Diefenbach,Kevin Einkauf,Jon Fallon,Jared Feldman,Kelsey K Finn,Pilar Garcia-Broncano,Ciputra Adijaya Hartana,Blake M Hauser,Chenyang Jiang,Paulina Kaplonek,Marshall Karpell,Eric C Koscher,Xiaodong Lian,Hang Liu,Jinqing Liu,Ngoc L Ly,Ashlin R Michell,Yelizaveta Rassadkina,Kyra Seiger,Libera Sessa,Sally Shin,Nishant Singh,Weiwei Sun,Xiaoming Sun,Hannah J Ticheli,Michael T Waring,Alex L Zhu,Galit Alter,Jonathan Z Li,Daniel Lingwood,Aaron G Schmidt,Mathias Lichterfeld,Bruce D Walker,Xu G Yu,Robert F Padera,Shiv Pillai

Cell 176:1113-1127.e16 PubMed30712867

2019

Pharmacological Targeting of STK19 Inhibits Oncogenic NRAS-Driven Melanomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chengqian Yin,Bo Zhu,Ting Zhang,Tongzheng Liu,Shuyang Chen,Yu Liu,Xin Li,Xiao Miao,Shanshan Li,Xia Mi,Jie Zhang,Li Li,Guo Wei,Zhi-Xiang Xu,Xiumei Gao,Canhua Huang,Zhi Wei,Colin R Goding,Peng Wang,Xianming Deng,Rutao Cui

Molecular and cellular biology 31:1066-75 PubMed21173159

2010

β-Arrestin 1 inhibits the GTPase-activating protein function of ARHGAP21, promoting activation of RhoA following angiotensin II type 1A receptor stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

D F Anthony,Y Y Sin,S Vadrevu,N Advant,J P Day,A M Byrne,M J Lynch,G Milligan,M D Houslay,G S Baillie
View all publications
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