Mouse Monoclonal BRAF antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BRAF aa 250-450.
Images
![Western blot - Anti-BRAF antibody [CL4004] (AB220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--western-blot-img93639.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRAF antibody [CL4004] (AB220032)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9970.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58827.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107498.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89005.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human BRAF aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link P15056
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Alternative names
Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF1, BRAF, RAFB1
Recommended products
Mouse Monoclonal BRAF antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BRAF aa 250-450.
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human BRAF aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link P15056
- Clone number
CL4004
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-BRAF antibody [CL4004] (ab220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--western-blot-img93639.jpg "Western blot - Anti-BRAF antibody [CL4004] (ab220032)")
Western blot - Anti-BRAF antibody [CL4004] (ab220032)
All lanes: Western blot - Anti-BRAF antibody [CL4004] (ab220032) at 1/500 dilution
All lanes: MOLT4 cell line lysate
Predicted band size: 84 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9970.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling B Raf with ab220032 at 1/500 dilution shows cytoplasmic immunoreactivity in seminiferous tubules.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58827.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human breast cancer tissue labeling B Raf with ab220032 at 1/500 dilution shows moderate cytoplasmic immunoreactivity in tumor cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107498.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human fallopian tube tissue labeling B Raf with ab220032 at 1/500 dilution shows strong cytoplasmic immunoreactivity in glandular cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-cl4004-ab220032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89005.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate cancer tissue labeling B Raf with ab220032 at 1/500 dilution shows moderate cytoplasmic immunoreactivity in tumor cells.
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