AB220032

Anti-BRAF antibody [CL4004]

Name: Anti-BRAF antibody [CL4004] (ab220032) | Abcam
Brand: Abcam Limited
SKU: AB220032

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(1 Publication)

Mouse Monoclonal BRAF antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BRAF aa 250-450.

View Alternative Names

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human breast cancer tissue labeling B Raf with ab220032 at 1/500 dilution shows moderate cytoplasmic immunoreactivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate cancer tissue labeling B Raf with ab220032 at 1/500 dilution shows moderate cytoplasmic immunoreactivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling B Raf with ab220032 at 1/500 dilution shows cytoplasmic immunoreactivity in seminiferous tubules.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (AB220032)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human fallopian tube tissue labeling B Raf with ab220032 at 1/500 dilution shows strong cytoplasmic immunoreactivity in glandular cells.

Supplier Data

Western blot - Anti-BRAF antibody [CL4004] (AB220032)

All lanes:

Western blot - Anti-BRAF antibody [CL4004] (ab220032) at 1/500 dilution

All lanes:

MOLT4 cell line lysate

Predicted band size: 84 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL4004

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human BRAF aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

P15056

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).

See full target information BRAF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:78 PubMed40000966

2025

A novel mechanism of FTO modulating the progression of endometriosis through mediating the m6A methylation of GEF-H1 in a YTHDF1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Ding,Hua-Ying Zhang,Jia-Hao Chen,Meng-Jie Yang,Zhi-Xiong Huang,Yi-Hong Lei,Qin-Kun Sun,Jian-Bin Bai,Dian-Chao Lin,Jian-Fa Lan,Lu-Lu Ren,Zheng-Yi Chen,Wei-Dong Zhou,Qiong-Hua Chen

View all publications

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Product promise

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