Rabbit Recombinant Monoclonal BRAF antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139316.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351)")
![Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--western-blot-img58154.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351)")
![Immunoprecipitation - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--immunoprecipitation-img21560.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71779.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351)")
![Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--western-blot-img3099.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (AB189351)")
Publications
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- International journal of cancer 133:590-62013Alternative splicing of BRAF transcripts and characterization of C-terminally truncated B-Raf isoforms in colorectal cancer.
- Clinical cancer research : an official journal of 18:5329-402012Oncogenic BRAF(V600E) promotes stromal cell-mediated immunosuppression via induction of interleukin-1 in melanoma.
- Cancer cell 19:652-632011c-Raf, but not B-Raf, is essential for development of K-Ras oncogene-driven non-small cell lung carcinoma.
- Neurology 73:1526-312009Alterations of BRAF and HIPK2 loci predominate in sporadic pilocytic astrocytoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Yu et. al.PubMed 19794125
- The Journal of clinical investigation 118:1739-492008BRAF gene duplication constitutes a mechanism of MAPK pathway activation in low-grade astrocytomas.Applications:WBReactive species:HumanStefan Pfister,Wibke G Janzarik,Marc Remke,Aurélie Ernst,Wiebke Werft,Natalia Becker,Grischa Toedt,Andrea Wittmann,Christian Kratz,Heike Olbrich,Rezvan Ahmadi,Barbara Thieme,Stefan Joos,Bernhard Radlwimmer,Andreas Kulozik,Torsten Pietsch,Christel Herold-Mende,Astrid Gnekow,Guido Reifenberger,Andrey Korshunov,Wolfram Scheurlen,Heymut Omran,Peter LichterPubMed 18398503
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Expected | Predicted |
| Rat | Predicted | Expected | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
2 products for Alternative Version
Target data
Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Alternative names
BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
Recommended products
Rabbit Recombinant Monoclonal BRAF antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP152Y
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab189351 is the carrier-free version of Anti-BRAF antibody [EP152Y] ab33899.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139316.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)
This IHC data was generated using the same anti-B Raf antibody clone, EP152Y, in a different buffer formulation (cat# Anti-BRAF antibody [EP152Y] ab33899).
Anti-BRAF antibody [EP152Y] ab33899 (unpurified) staining B Raf cells from human prostate tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed and permeabilized in PBS-Tween 20 prior to blocking in 70% serum for 10 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 1 hour at 25°C. A biotin conjugated goat polyclonal to mouse Ig was used as the secondary.
![Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--western-blot-img58154.jpg "Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)")
Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)
This data was developed using the same antibody clone in a different buffer formulation (Anti-BRAF antibody [EP152Y] ab33899).
Lanes 1- 2: Merged signal (red and green). Green - Anti-BRAF antibody [EP152Y] ab33899 observed at 90 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-BRAF antibody [EP152Y] ab33899 was shown to react with BRAF in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human BRAF knockout HeLa cell line ab265373 (knockout cell lysate Human BRAF knockout HeLa cell lysate ab257078) was used. Wild-type HeLa and BRAF knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-BRAF antibody [EP152Y] ab33899 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-BRAF antibody [EP152Y] (Anti-BRAF antibody [EP152Y] ab33899) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: BRAF knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 90 kDa
![Immunoprecipitation - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--immunoprecipitation-img21560.jpg "Immunoprecipitation - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)")
Immunoprecipitation - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)
Anti-BRAF antibody [EP152Y] ab33899 (Purified) at 1:500 dilution (0.954 μg/ml) immunoprecipitating BRAF in HeLa whole cell lysate .
Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2 (+): Anti-BRAF antibody [EP152Y] ab33899 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-BRAF antibody [EP152Y] ab33899 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST .This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189351)All lanes: Immunoprecipitation - Anti-BRAF antibody [EP152Y] (Anti-BRAF antibody [EP152Y] ab33899)
Predicted band size: 84 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71779.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling BRAF with purified Anti-BRAF antibody [EP152Y] ab33899 at 1/100 dilution (4.77 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BRAF antibody [EP152Y] ab33899).
![Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--western-blot-img3099.jpg "Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)")
Western blot - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)
Lanes 1 - 3: Merged signal (red and green). Green - Anti-BRAF antibody [EP152Y] ab33899 (unpurified) observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.Anti-BRAF antibody [EP152Y] ab33899 was shown to recognize BRAF in wild-type HAP1 cells as signal was lost at the expected MW in BRAF knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BRAF knockout samples were subjected to SDS-PAGE. Anti-BRAF antibody [EP152Y] ab33899 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BRAF antibody [EP152Y] ab33899).
All lanes: Western blot - Anti-BRAF antibody [EP152Y] (Anti-BRAF antibody [EP152Y] ab33899) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 40 µg
Lane 2: BRAF knockout HAP1 whole cell lysate at 40 µg
Lane 3: HeLa whole cell lysate at 40 µg
Predicted band size: 84 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8120.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)
This IHC data was generated using the same anti-B Raf antibody clone, EP152Y, in a different buffer formulation (cat# Anti-BRAF antibody [EP152Y] ab33899).
This image shows paraffin embedded human prostate cancer tissue sample stained with Anti-BRAF antibody [EP152Y] ab33899 (unpurified) at 1/250 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-ep152y-bsa-and-azide-free-ab189351--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8120.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)")
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