Rat Monoclonal BRAF antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [pBR1] (AB183337), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-pbr1-ab183337--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86231.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [pBR1] (AB183337)")
Publications
Filter by
- Cancer 119:2765-702013Mutation-specific antibody detects mutant BRAFV600E protein expression in human colon carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrank A Sinicrope,Thomas C Smyrk,David Tougeron,Stephen N Thibodeau,Shalini Singh,Andrea Muranyi,Kandavel Shanmugam,Thomas M Grogan,Steven R Alberts,Qian ShiPubMed 23657789
Key facts
- Isotype
IgG2a
- Host species
Rat
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Boil tissue section in EDTA buffer pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
Associated Products
Select an associated product type
10 products for Alternative Product
Target data
Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Alternative names
Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF1, BRAF, RAFB1
Recommended products
Rat Monoclonal BRAF antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
IgG2a
- Host species
Rat
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
pBR1
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Protein A/G purified.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [pBR1] (ab183337), expandable thumbnail](https://content.abcam.com/products/images/braf-antibody-pbr1-ab183337--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86231.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [pBR1] (ab183337)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [pBR1] (ab183337)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling B Raf with ab183337 at 1/100 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com