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Rabbit Recombinant Monoclonal BRAF phospho S729 antibody. Suitable for WB and reacts with Rat samples. Cited in 10 publications.

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Images

Western blot - Anti-BRAF (phospho S729) antibody [EPR2207] (AB124794), expandable thumbnail
  • OI-RD Scanning - Anti-BRAF (phospho S729) antibody [EPR2207] (AB124794), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Not recommended
Not recommended
Predicted
Not recommended
Mouse
Not recommended
Not recommended
Predicted
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal BRAF phospho S729 antibody. Suitable for WB and reacts with Rat samples. Cited in 10 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR2207

Purification technique

Affinity purification Protein A

Specificity

Detects B Raf only when phosphorylated on serine 729.

Dissociation constant

9 x 10-12 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-BRAF (phospho S729) antibody [EPR2207] (ab124794), expandable thumbnail

    Western blot - Anti-BRAF (phospho S729) antibody [EPR2207] (ab124794)

    All lanes: Western blot - Anti-BRAF (phospho S729) antibody [EPR2207] (ab124794) at 1/1000 dilution

    Lane 1: PC-12 cell lysates (untreated) at 10 µg

    Lane 2: PC-12 cell lysates treated with Lambda Phosphatase at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 84 kDa

  • OI-RD Scanning - Anti-BRAF (phospho S729) antibody [EPR2207] (ab124794), expandable thumbnail

    OI-RD Scanning - Anti-BRAF (phospho S729) antibody [EPR2207] (ab124794)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com