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AB124794

Anti-BRAF (phospho S729) antibody [EPR2207]

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(13 Publications)

Rabbit Recombinant Monoclonal BRAF phospho S729 antibody. Suitable for WB and reacts with Rat samples. Cited in 13 publications.

View Alternative Names

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

2 Images
Western blot - Anti-BRAF (phospho S729) antibody [EPR2207] (AB124794)
  • WB

Unknown

Western blot - Anti-BRAF (phospho S729) antibody [EPR2207] (AB124794)

All lanes:

Western blot - Anti-BRAF (phospho S729) antibody [EPR2207] (ab124794) at 1/1000 dilution

Lane 1:

PC-12 cell lysates (untreated) at 10 µg

Lane 2:

PC-12 cell lysates treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 84 kDa

false

OI-RD Scanning - Anti-BRAF (phospho S729) antibody [EPR2207] (AB124794)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-BRAF (phospho S729) antibody [EPR2207] (AB124794)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-BRAF (phospho S729) antibody [EPR2207] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2207

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Detects B Raf only when phosphorylated on serine 729.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).
See full target information BRAF phospho S729
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 21:2094-2106 PubMed39433876

2024

Multiplexed profiling of intracellular protein abundance, activity, interactions and druggability with LABEL-seq.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica J Simon,Douglas M Fowler,Dustin J Maly

Communications biology 7:1073 PubMed39217250

2024

Reversible control of kinase signaling through chemical-induced dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Sun,Rihong Zhou,Jin Hu,Shan Feng,Qi Hu

Cellular and molecular life sciences : CMLS 80:61 PubMed36763212

2023

Co-dependent regulation of p-BRAF and potassium channel KCNMA1 levels drives glioma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Xie,Chengyan Xu,Cheng Wu,Yuhan Lou,Jingwei Duan,Rong Sang,Ziwei Lou,Jiaru Hou,Wanzhong Ge,Yongmei Xi,Xiaohang Yang

Nature communications 13:7343 PubMed36446791

2022

HSP90-CDC37-PP5 forms a structural platform for kinase dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmeen Oberoi,Xavi Aran Guiu,Emily A Outwin,Pascale Schellenberger,Theodoros I Roumeliotis,Jyoti S Choudhary,Laurence H Pearl

Nature 609:416-423 PubMed35830882

2022

Structure of the MRAS-SHOC2-PP1C phosphatase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary J Hauseman,Michelle Fodor,Anxhela Dhembi,Jessica Viscomi,David Egli,Melusine Bleu,Stephanie Katz,Eunyoung Park,Dong Man Jang,Kathryn A Porter,Fabian Meili,Hongqiu Guo,Grainne Kerr,Sandra Mollé,Camilo Velez-Vega,Kim S Beyer,Giorgio G Galli,Saveur-Michel Maira,Travis Stams,Kirk Clark,Michael J Eck,Luca Tordella,Claudio R Thoma,Daniel A King

Autophagy 17:2128-2143 PubMed32866426

2020

Keratinocyte autophagy enables the activation of keratinocytes and fibroblastsand facilitates wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Qiang,Seungwon Yang,Yan-Hong Cui,Yu-Ying He

The Journal of biological chemistry 295:2407-2420 PubMed31929109

2020

Analyses of the oncogenic BRAF variant reveal a kinase-independent function of BRAF in activating MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Cope,Borna Novak,Zhiwei Liu,Maria Cavallo,Amber Y Gunderwala,Matthew Connolly,Zhihong Wang

Acta pharmaceutica Sinica. B 9:294-303 PubMed30976491

2019

Glycyrrhetinic acid binds to the conserved P-loop region and interferes with the interaction of RAS-effector proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Zhihua Wang,Xiaoyao Ma,Shengnan Yang,Xueyan Hu,Jin Tao,Yuanyuan Hou,Gang Bai

Developmental cell 48:345-360.e7 PubMed30595535

2019

AMPK Promotes SPOP-Mediated NANOG Degradation to Regulate Prostate Cancer Cell Stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Xinbo Wang,Jiali Jin,Fangning Wan,Linlin Zhao,Hongshang Chu,Cong Chen,Guanghong Liao,Jian Liu,Yue Yu,Hongqi Teng,Lan Fang,Cong Jiang,Weijuan Pan,Xin Xie,Jia Li,Xiaolin Lu,Xuejun Jiang,Xin Ge,Dingwei Ye,Ping Wang

Cell reports 25:1501-1510.e3 PubMed30404005

2018

BRAF Splice Variant Resistance to RAF Inhibitor Requires Enhanced MEK Association.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Vido,Kaitlyn Le,Edward J Hartsough,Andrew E Aplin
View all publications
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Product promise

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