AB68215

Anti-BRAF (phospho T401) antibody [EPR2208Y]

Name: Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215) | Abcam
Brand: Abcam Limited
SKU: AB68215
Price: 325 USD
Availability: InStock
Rating: 5 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(8 Publications)

Rabbit Recombinant Monoclonal BRAF phospho T401 antibody. Suitable for WB and reacts with Rat samples. Cited in 8 publications.

View Alternative Names

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

1 Images

Unknown

Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (AB68215)

All lanes:

Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215) at 1/5000 dilution

Lane 1:

PC-12 cell lysates (cells were untreated) at 10 µg

Lane 2:

PC-12 cell lysates (cells were treated with TPA) at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 84 kDa

Observed band size: 85 kDa

false

Carrier free

Anti-BRAF (phospho T401) antibody [EPR2208Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2208Y

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects B Raf phosphorylated on threonine 401.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).

See full target information BRAF pT401

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1073 PubMed39217250

2024

Reversible control of kinase signaling through chemical-induced dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Sun,Rihong Zhou,Jin Hu,Shan Feng,Qi Hu

American journal of cancer research 13:638-653 PubMed36895965

2023

FOXA2 and STAT5A regulate oncogenic activity of KIF5B-RET fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Ran Lee,Jung-Young Shin,Min-Young Kim,Jeong-Oh Kim,Chan Kwon Jung,Jinhyoung Kang

Communications biology 4:688 PubMed34099862

2021

REG3A/REG3B promotes acinar to ductal metaplasia through binding to EXTL3 and activating the RAS-RAF-MEK-ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huairong Zhang,Andrea Liliam Gomez Corredor,Julia Messina-Pacheco,Qing Li,George Zogopoulos,Nancy Kaddour,Yifan Wang,Bing-Yin Shi,Alex Gregorieff,Jun-Li Liu,Zu-Hua Gao

BMC gastroenterology 21:86 PubMed33622273

2021

V-Raf murine sarcoma viral oncogene homolog B1 (BRAF) as a prognostic biomarker of poor outcomes in esophageal cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Aihemaijiang Kuerbanjiang,Maimaitiyiming Maimaituerxun,Yanjun Zhang,Yiliang Li,Gang Cui,Aibaidula Abuduhabaier,Abuduwaili Aierken,Buya Miranbieke,Meilikezati Anzaer,Yusufu Maimaiti

Acta pharmaceutica Sinica. B 9:294-303 PubMed30976491

2019

Glycyrrhetinic acid binds to the conserved P-loop region and interferes with the interaction of RAS-effector proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Zhihua Wang,Xiaoyao Ma,Shengnan Yang,Xueyan Hu,Jin Tao,Yuanyuan Hou,Gang Bai

Oncology letters 15:6225-6232 PubMed29849782

2018

Focal adhesion kinase promotes progression and predicts poor clinical outcomes in patients with osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Jie Gu,Bin Zhou

Neuroscience 372:192-212 PubMed29292072

2018

The GABAα5-selective Modulator, RO4938581, Rescues Protein Anomalies in the Ts65Dn Mouse Model of Down Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

A Block,Md M Ahmed,N Rueda,M-C Hernandez,C Martinez-Cué,K J Gardiner

Journal of cellular biochemistry 119:2864-2874 PubMed29073728

2017

HOXA3 promotes tumor growth of human colon cancer through activating EGFR/Ras/Raf/MEK/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xianxiang Zhang,Guangwei Liu,Lei Ding,Tao Jiang,Shihong Shao,Yuan Gao,Yun Lu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRAF (phospho T401) antibody [EPR2208Y]

Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (AB68215)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Product promise