Rabbit Recombinant Monoclonal BRAF phospho T401 antibody. Suitable for WB and reacts with Rat samples. Cited in 7 publications.
Images
![Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (AB68215), expandable thumbnail](https://content.abcam.com/products/images/braf-phospho-t401-antibody-epr2208y-ab68215--western-blot-img49472.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (AB68215)")
Publications
Filter by
- American journal of cancer research 13:638-6532023FOXA2 and STAT5A regulate oncogenic activity of KIF5B-RET fusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi-Ran Lee,Jung-Young Shin,Min-Young Kim,Jeong-Oh Kim,Chan Kwon Jung,Jinhyoung KangPubMed 36895965
- Communications biology 4:6882021REG3A/REG3B promotes acinar to ductal metaplasia through binding to EXTL3 and activating the RAS-RAF-MEK-ERK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuairong Zhang,Andrea Liliam Gomez Corredor,Julia Messina-Pacheco,Qing Li,George Zogopoulos,Nancy Kaddour,Yifan Wang,Bing-Yin Shi,Alex Gregorieff,Jun-Li Liu,Zu-Hua GaoPubMed 34099862
- BMC gastroenterology 21:862021V-Raf murine sarcoma viral oncogene homolog B1 (BRAF) as a prognostic biomarker of poor outcomes in esophageal cancer patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAihemaijiang Kuerbanjiang,Maimaitiyiming Maimaituerxun,Yanjun Zhang,Yiliang Li,Gang Cui,Aibaidula Abuduhabaier,Abuduwaili Aierken,Buya Miranbieke,Meilikezati Anzaer,Yusufu MaimaitiPubMed 33622273
- Acta pharmaceutica Sinica. B 9:294-3032019Glycyrrhetinic acid binds to the conserved P-loop region and interferes with the interaction of RAS-effector proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Zhang,Zhihua Wang,Xiaoyao Ma,Shengnan Yang,Xueyan Hu,Jin Tao,Yuanyuan Hou,Gang BaiPubMed 30976491
- Oncology letters 15:6225-62322018Focal adhesion kinase promotes progression and predicts poor clinical outcomes in patients with osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua-Jie Gu et. al.PubMed 29849782
- Neuroscience 372:192-2122018The GABAα5-selective Modulator, RO4938581, Rescues Protein Anomalies in the Ts65Dn Mouse Model of Down Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Block,Md M Ahmed,N Rueda,M-C Hernandez,C Martinez-Cué,K J GardinerPubMed 29292072
- Journal of cellular biochemistry 119:2864-28742017HOXA3 promotes tumor growth of human colon cancer through activating EGFR/Ras/Raf/MEK/ERK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianxiang Zhang,Guangwei Liu,Lei Ding,Tao Jiang,Shihong Shao,Yuan Gao,Yun LuPubMed 29073728
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Predicted | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Tested | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Alternative names
Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF1, BRAF, RAFB1
Recommended products
Rabbit Recombinant Monoclonal BRAF phospho T401 antibody. Suitable for WB and reacts with Rat samples. Cited in 7 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR2208Y
- Purification technique
Affinity purification Protein A
- Specificity
This antibody detects B Raf phosphorylated on threonine 401.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215), expandable thumbnail](https://content.abcam.com/products/images/braf-phospho-t401-antibody-epr2208y-ab68215--western-blot-img49472.jpg "Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)")
Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)
All lanes: Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215) at 1/5000 dilution
Lane 1: PC-12 cell lysates (cells were untreated) at 10 µg
Lane 2: PC-12 cell lysates (cells were treated with TPA) at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 85 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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