Rabbit Polyclonal BRAF phospho T599 antibody. Suitable for ICC, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BRAF phospho T599 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human BRAF phospho T599 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P15056
Reactivity data
Select an application| ICC | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Alternative names
Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, RAFB1, BRAF1, BRAF
Recommended products
Rabbit Polyclonal BRAF phospho T599 antibody. Suitable for ICC, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BRAF phospho T599 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human BRAF phospho T599 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P15056
- Purification technique
Affinity purification Immunogen
- Specificity
ab192813 detects endogenous levels of B Raf only when phosphorylated at threonine 599.
- Concentration
- Purification notes
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
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Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry - Anti-BRAF (phospho T599) antibody (ab192813)
Immunofluorescence staining of methanol-fixed HeLa cells using ab192813
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (phospho T599) antibody (ab192813)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling B Raf (phospho T599) using ab192813 at a 1/50 dilution.
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