AB192813

Anti-BRAF (phospho T599) antibody

Name: Anti-BRAF (phospho T599) antibody (ab192813) | Abcam
Brand: Abcam Limited
SKU: AB192813
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal BRAF phospho T599 antibody. Suitable for ICC, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BRAF phospho T599 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

2 Images

Unknown

Immunocytochemistry - Anti-BRAF (phospho T599) antibody (AB192813)

Immunofluorescence staining of methanol-fixed HeLa cells using ab192813

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (phospho T599) antibody (AB192813)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (phospho T599) antibody (AB192813)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling B Raf (phospho T599) using ab192813 at a 1/50 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Synthetic Peptide within Human BRAF phospho T599 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P15056

Specificity

ab192813 detects endogenous levels of B Raf only when phosphorylated at threonine 599.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/200", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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Product details

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).

See full target information BRAF phospho T599

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:1009 PubMed38307859

2024

Targeting ALK averts ribonuclease 1-induced immunosuppression and enhances antitumor immunity in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiao Liu,Chenhao Zhou,Weiya Xia,Yifan Zhou,Yufan Qiu,Jialei Weng,Qiang Zhou,Wanyong Chen,Ying-Nai Wang,Heng-Huan Lee,Shao-Chun Wang,Ming Kuang,Dihua Yu,Ning Ren,Mien-Chie Hung

View all publications

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